Abstract
A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.
| Original language | English |
|---|---|
| Pages (from-to) | 2095-2105 |
| Number of pages | 11 |
| Journal | Biomaterials |
| Volume | 22 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Aug 1 2001 |
Fingerprint
Keywords
- Alkaline phosphatase
- Bone matrix protein
- Calcium
- Hyaluronic acid polymer
- Mineralization
- Stromal cells
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold. / Lisignoli, G.; Zini, N.; Remiddi, G.; Piacentini, A.; Puggioli, A.; Trimarchi, C.; Fini, M.; Maraldi, N. M.; Facchini, A.
In: Biomaterials, Vol. 22, No. 15, 01.08.2001, p. 2095-2105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold
AU - Lisignoli, G.
AU - Zini, N.
AU - Remiddi, G.
AU - Piacentini, A.
AU - Puggioli, A.
AU - Trimarchi, C.
AU - Fini, M.
AU - Maraldi, N. M.
AU - Facchini, A.
PY - 2001/8/1
Y1 - 2001/8/1
N2 - A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.
AB - A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.
KW - Alkaline phosphatase
KW - Bone matrix protein
KW - Calcium
KW - Hyaluronic acid polymer
KW - Mineralization
KW - Stromal cells
UR - http://www.scopus.com/inward/record.url?scp=0035423786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035423786&partnerID=8YFLogxK
U2 - 10.1016/S0142-9612(00)00398-7
DO - 10.1016/S0142-9612(00)00398-7
M3 - Article
C2 - 11432589
AN - SCOPUS:0035423786
VL - 22
SP - 2095
EP - 2105
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 15
ER -