Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold

G. Lisignoli, N. Zini, G. Remiddi, A. Piacentini, A. Puggioli, C. Trimarchi, M. Fini, N. M. Maraldi, A. Facchini

Research output: Contribution to journalArticlepeer-review

Abstract

A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

Original languageEnglish
Pages (from-to)2095-2105
Number of pages11
JournalBiomaterials
Volume22
Issue number15
DOIs
Publication statusPublished - Aug 1 2001

Keywords

  • Alkaline phosphatase
  • Bone matrix protein
  • Calcium
  • Hyaluronic acid polymer
  • Mineralization
  • Stromal cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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