Abstract
A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.
Original language | English |
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Pages (from-to) | 2095-2105 |
Number of pages | 11 |
Journal | Biomaterials |
Volume | 22 |
Issue number | 15 |
DOIs | |
Publication status | Published - Aug 1 2001 |
Keywords
- Alkaline phosphatase
- Bone matrix protein
- Calcium
- Hyaluronic acid polymer
- Mineralization
- Stromal cells
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering