Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold

G. Lisignoli, N. Zini, G. Remiddi, A. Piacentini, A. Puggioli, C. Trimarchi, M. Fini, N. M. Maraldi, A. Facchini

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

Original languageEnglish
Pages (from-to)2095-2105
Number of pages11
JournalBiomaterials
Volume22
Issue number15
DOIs
Publication statusPublished - Aug 1 2001

Fingerprint

Hyaluronic acid
Fibroblast Growth Factor 2
Hyaluronic Acid
Fibroblasts
Mesenchymal Stromal Cells
Scaffolds
Osteopontin
Alkaline Phosphatase
Rats
Polymers
Bone
Phosphatases
Cells
Integrin-Binding Sialoprotein
Electron microscopy
Electron Microscopy
Calcium
Tolonium Chloride
Bone Development
Osteocalcin

Keywords

  • Alkaline phosphatase
  • Bone matrix protein
  • Calcium
  • Hyaluronic acid polymer
  • Mineralization
  • Stromal cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold. / Lisignoli, G.; Zini, N.; Remiddi, G.; Piacentini, A.; Puggioli, A.; Trimarchi, C.; Fini, M.; Maraldi, N. M.; Facchini, A.

In: Biomaterials, Vol. 22, No. 15, 01.08.2001, p. 2095-2105.

Research output: Contribution to journalArticle

Lisignoli, G. ; Zini, N. ; Remiddi, G. ; Piacentini, A. ; Puggioli, A. ; Trimarchi, C. ; Fini, M. ; Maraldi, N. M. ; Facchini, A. / Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold. In: Biomaterials. 2001 ; Vol. 22, No. 15. pp. 2095-2105.
@article{651808fdcf1f4fd8a16375042c35404a,
title = "Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold",
abstract = "A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff{\circledR} 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff{\circledR} 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff{\circledR} 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.",
keywords = "Alkaline phosphatase, Bone matrix protein, Calcium, Hyaluronic acid polymer, Mineralization, Stromal cells",
author = "G. Lisignoli and N. Zini and G. Remiddi and A. Piacentini and A. Puggioli and C. Trimarchi and M. Fini and Maraldi, {N. M.} and A. Facchini",
year = "2001",
month = "8",
day = "1",
doi = "10.1016/S0142-9612(00)00398-7",
language = "English",
volume = "22",
pages = "2095--2105",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "15",

}

TY - JOUR

T1 - Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold

AU - Lisignoli, G.

AU - Zini, N.

AU - Remiddi, G.

AU - Piacentini, A.

AU - Puggioli, A.

AU - Trimarchi, C.

AU - Fini, M.

AU - Maraldi, N. M.

AU - Facchini, A.

PY - 2001/8/1

Y1 - 2001/8/1

N2 - A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

AB - A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff® 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff® 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type I. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff® 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

KW - Alkaline phosphatase

KW - Bone matrix protein

KW - Calcium

KW - Hyaluronic acid polymer

KW - Mineralization

KW - Stromal cells

UR - http://www.scopus.com/inward/record.url?scp=0035423786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035423786&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(00)00398-7

DO - 10.1016/S0142-9612(00)00398-7

M3 - Article

C2 - 11432589

AN - SCOPUS:0035423786

VL - 22

SP - 2095

EP - 2105

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 15

ER -