Basic fibroblast growth factor messenger ribonucleic acid levels in eutopic and ectopic human endometrial stromal cells as assessed by competitive polymerase chain reaction amplification

Anna Maria Di Blasio, Giovanna Centinaio, Cristiana Carniti, Edgardo Somigliana, Paola Vigaho, Mario Vignali

Research output: Contribution to journalArticle

Abstract

Previous studies have localized basic fibroblast growth factor (bFGF) and its mRNA in normal and neoplastic endometrium but the expression of bGFG mRNA in endometriosis cells is virtually unknown. Our purpose was to investigate the presence of bFGF and its receptor mRNAs in highly purified primary cultures of stromal cells isolated from six eutopic endometrial samples obtained from patients without evidence of endometriosis and five endometriosis cyst linings. Using reverse transcriptase-polymerase chain reaction (RT-PCR), single major DNA bands of the expected sizes for bFGF and its receptor (354 and 661 bp, respectively) were detected in both endometrial and endometriosis samples. A competitive RT-PCR, that uses coprimer extension and amplification of a bovine RNA as an internal standard, was developed for semiquantitative estimation of bFGF gene expression. The target to standard mean ratios ± SEM in six normal endometrial samples and in five endometriosis cultures were 26.7 ± 10.7 and 9.2 ± 3.0, respectively. Furthermore, when data were analyzed according to the cyst diameter, levels of bFGF mRNA resulted statistically higher (P <0.05) in bigger cysts when compared to those detected in smaller ones (16 ± 2.7 and 4.7 ± 1.8, respectively). These results demonstrate that the genes coding for bFGF and its receptor are expressed in endometriosis cells, but levels of bFGF mRNA are generally lower than those detected in their eutopic counterpart. Moreover, they indicate that endometriosis cells derived from large cysts have increased bFGF mRNA levels. Thus, bFGF could be one of the factors responsible for a more or less active behavior of the endometnotic lesion.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume115
Issue number2
DOIs
Publication statusPublished - Dec 29 1995

Fingerprint

Polymerase chain reaction
Fibroblast Growth Factor 2
Stromal Cells
Amplification
Endometriosis
RNA
Polymerase Chain Reaction
Fibroblast Growth Factor Receptors
Messenger RNA
Cysts
RNA-Directed DNA Polymerase
Reverse Transcriptase Polymerase Chain Reaction
Endometrium
Linings
Gene expression
Genes
Gene Expression
Scanning electron microscopy
DNA

Keywords

  • Basic fibroblast growth factor
  • Competitive polymerase chain reaction
  • Endometrial cell
  • Endometriosis cell

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

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title = "Basic fibroblast growth factor messenger ribonucleic acid levels in eutopic and ectopic human endometrial stromal cells as assessed by competitive polymerase chain reaction amplification",
abstract = "Previous studies have localized basic fibroblast growth factor (bFGF) and its mRNA in normal and neoplastic endometrium but the expression of bGFG mRNA in endometriosis cells is virtually unknown. Our purpose was to investigate the presence of bFGF and its receptor mRNAs in highly purified primary cultures of stromal cells isolated from six eutopic endometrial samples obtained from patients without evidence of endometriosis and five endometriosis cyst linings. Using reverse transcriptase-polymerase chain reaction (RT-PCR), single major DNA bands of the expected sizes for bFGF and its receptor (354 and 661 bp, respectively) were detected in both endometrial and endometriosis samples. A competitive RT-PCR, that uses coprimer extension and amplification of a bovine RNA as an internal standard, was developed for semiquantitative estimation of bFGF gene expression. The target to standard mean ratios ± SEM in six normal endometrial samples and in five endometriosis cultures were 26.7 ± 10.7 and 9.2 ± 3.0, respectively. Furthermore, when data were analyzed according to the cyst diameter, levels of bFGF mRNA resulted statistically higher (P <0.05) in bigger cysts when compared to those detected in smaller ones (16 ± 2.7 and 4.7 ± 1.8, respectively). These results demonstrate that the genes coding for bFGF and its receptor are expressed in endometriosis cells, but levels of bFGF mRNA are generally lower than those detected in their eutopic counterpart. Moreover, they indicate that endometriosis cells derived from large cysts have increased bFGF mRNA levels. Thus, bFGF could be one of the factors responsible for a more or less active behavior of the endometnotic lesion.",
keywords = "Basic fibroblast growth factor, Competitive polymerase chain reaction, Endometrial cell, Endometriosis cell",
author = "{Di Blasio}, {Anna Maria} and Giovanna Centinaio and Cristiana Carniti and Edgardo Somigliana and Paola Vigaho and Mario Vignali",
year = "1995",
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T1 - Basic fibroblast growth factor messenger ribonucleic acid levels in eutopic and ectopic human endometrial stromal cells as assessed by competitive polymerase chain reaction amplification

AU - Di Blasio, Anna Maria

AU - Centinaio, Giovanna

AU - Carniti, Cristiana

AU - Somigliana, Edgardo

AU - Vigaho, Paola

AU - Vignali, Mario

PY - 1995/12/29

Y1 - 1995/12/29

N2 - Previous studies have localized basic fibroblast growth factor (bFGF) and its mRNA in normal and neoplastic endometrium but the expression of bGFG mRNA in endometriosis cells is virtually unknown. Our purpose was to investigate the presence of bFGF and its receptor mRNAs in highly purified primary cultures of stromal cells isolated from six eutopic endometrial samples obtained from patients without evidence of endometriosis and five endometriosis cyst linings. Using reverse transcriptase-polymerase chain reaction (RT-PCR), single major DNA bands of the expected sizes for bFGF and its receptor (354 and 661 bp, respectively) were detected in both endometrial and endometriosis samples. A competitive RT-PCR, that uses coprimer extension and amplification of a bovine RNA as an internal standard, was developed for semiquantitative estimation of bFGF gene expression. The target to standard mean ratios ± SEM in six normal endometrial samples and in five endometriosis cultures were 26.7 ± 10.7 and 9.2 ± 3.0, respectively. Furthermore, when data were analyzed according to the cyst diameter, levels of bFGF mRNA resulted statistically higher (P <0.05) in bigger cysts when compared to those detected in smaller ones (16 ± 2.7 and 4.7 ± 1.8, respectively). These results demonstrate that the genes coding for bFGF and its receptor are expressed in endometriosis cells, but levels of bFGF mRNA are generally lower than those detected in their eutopic counterpart. Moreover, they indicate that endometriosis cells derived from large cysts have increased bFGF mRNA levels. Thus, bFGF could be one of the factors responsible for a more or less active behavior of the endometnotic lesion.

AB - Previous studies have localized basic fibroblast growth factor (bFGF) and its mRNA in normal and neoplastic endometrium but the expression of bGFG mRNA in endometriosis cells is virtually unknown. Our purpose was to investigate the presence of bFGF and its receptor mRNAs in highly purified primary cultures of stromal cells isolated from six eutopic endometrial samples obtained from patients without evidence of endometriosis and five endometriosis cyst linings. Using reverse transcriptase-polymerase chain reaction (RT-PCR), single major DNA bands of the expected sizes for bFGF and its receptor (354 and 661 bp, respectively) were detected in both endometrial and endometriosis samples. A competitive RT-PCR, that uses coprimer extension and amplification of a bovine RNA as an internal standard, was developed for semiquantitative estimation of bFGF gene expression. The target to standard mean ratios ± SEM in six normal endometrial samples and in five endometriosis cultures were 26.7 ± 10.7 and 9.2 ± 3.0, respectively. Furthermore, when data were analyzed according to the cyst diameter, levels of bFGF mRNA resulted statistically higher (P <0.05) in bigger cysts when compared to those detected in smaller ones (16 ± 2.7 and 4.7 ± 1.8, respectively). These results demonstrate that the genes coding for bFGF and its receptor are expressed in endometriosis cells, but levels of bFGF mRNA are generally lower than those detected in their eutopic counterpart. Moreover, they indicate that endometriosis cells derived from large cysts have increased bFGF mRNA levels. Thus, bFGF could be one of the factors responsible for a more or less active behavior of the endometnotic lesion.

KW - Basic fibroblast growth factor

KW - Competitive polymerase chain reaction

KW - Endometrial cell

KW - Endometriosis cell

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