Bax is necessary for PGC1α pro-apoptotic effect in colorectal cancer cells

Ilenia D'Errico; Giuseppe Lo Sasso; Lorena Salvatore; Stefania Murzilli; Nicola Martelli; Maricarmen Cristofaro; Dominga Latorre; Gaetano Villani; Antonio Moschetta

doi:10.4161/cc.10.17.16791

Bax is necessary for PGC1α pro-apoptotic effect in colorectal cancer cells

Ilenia D'Errico, Giuseppe Lo Sasso, Lorena Salvatore, Stefania Murzilli, Nicola Martelli, Maricarmen Cristofaro, Dominga Latorre, Gaetano Villani, Antonio Moschetta

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article

Abstract

We have recently shown that the transcriptional coactivator PGC1α, a master regulator of mitochondrial biogenesis and function, is involved in the control of the intestinal epithelium cell fate. Furthermore, PGC1α protects against colon cancer formation by promoting ROS accumulation and, consequently, mitochondria-mediated apoptosis. Here we provide an additional mechanistic insight into the tumor suppressor activity of PGC1α showing that its pro-apoptotic effect is mediated by Bax. In fact, PGC1α overexpression in HCT116 Bax ^-/- colorectal cancer cells stimulates mitochondrial production and activity, but it fails to induce cell death as well as to oppose tumor growth in the xenograft model. The lack of ROS accumulation in the Bax ^-/- cells strengthens our view that the PGC1α-induced oxidative burst represents one of the main apoptosis-driving factors in colorectal cancer cells.

Original language	English
Pages (from-to)	2937-2945
Number of pages	9
Journal	Cell Cycle
Volume	10
Issue number	17
DOIs	https://doi.org/10.4161/cc.10.17.16791
Publication status	Published - Sep 1 2011

Keywords

Apoptosis
Intestine
Mitochondria
Nuclear receptors
Reactive oxygen species (ROS)

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Developmental Biology

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D'Errico, I., Lo Sasso, G., Salvatore, L., Murzilli, S., Martelli, N., Cristofaro, M., Latorre, D., Villani, G., & Moschetta, A. (2011). Bax is necessary for PGC1α pro-apoptotic effect in colorectal cancer cells. Cell Cycle, 10(17), 2937-2945. https://doi.org/10.4161/cc.10.17.16791

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abstract = "We have recently shown that the transcriptional coactivator PGC1α, a master regulator of mitochondrial biogenesis and function, is involved in the control of the intestinal epithelium cell fate. Furthermore, PGC1α protects against colon cancer formation by promoting ROS accumulation and, consequently, mitochondria-mediated apoptosis. Here we provide an additional mechanistic insight into the tumor suppressor activity of PGC1α showing that its pro-apoptotic effect is mediated by Bax. In fact, PGC1α overexpression in HCT116 Bax -/- colorectal cancer cells stimulates mitochondrial production and activity, but it fails to induce cell death as well as to oppose tumor growth in the xenograft model. The lack of ROS accumulation in the Bax -/- cells strengthens our view that the PGC1α-induced oxidative burst represents one of the main apoptosis-driving factors in colorectal cancer cells.",

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T1 - Bax is necessary for PGC1α pro-apoptotic effect in colorectal cancer cells

AU - D'Errico, Ilenia

AU - Lo Sasso, Giuseppe

AU - Salvatore, Lorena

AU - Murzilli, Stefania

AU - Martelli, Nicola

AU - Cristofaro, Maricarmen

AU - Latorre, Dominga

AU - Villani, Gaetano

AU - Moschetta, Antonio

PY - 2011/9/1

Y1 - 2011/9/1

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AB - We have recently shown that the transcriptional coactivator PGC1α, a master regulator of mitochondrial biogenesis and function, is involved in the control of the intestinal epithelium cell fate. Furthermore, PGC1α protects against colon cancer formation by promoting ROS accumulation and, consequently, mitochondria-mediated apoptosis. Here we provide an additional mechanistic insight into the tumor suppressor activity of PGC1α showing that its pro-apoptotic effect is mediated by Bax. In fact, PGC1α overexpression in HCT116 Bax -/- colorectal cancer cells stimulates mitochondrial production and activity, but it fails to induce cell death as well as to oppose tumor growth in the xenograft model. The lack of ROS accumulation in the Bax -/- cells strengthens our view that the PGC1α-induced oxidative burst represents one of the main apoptosis-driving factors in colorectal cancer cells.

KW - Apoptosis

KW - Intestine

KW - Mitochondria

KW - Nuclear receptors

KW - Reactive oxygen species (ROS)

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