BCG-infected adherent mononuclear cells release cytokines that regulate group 1 CD1 molecule expression

Salvatore Pasquale Prete, Anna Giuliani, Stefania D'Atri, Grazia Graziani, Alessandra Balduzzi, Marco Rinaldo Oggioni, Elisabetta Iona, Giampiero Girolomoni, Laura Bonmassar, Luigina Romani, Ornella Franzese

Research output: Contribution to journalArticle

Abstract

Increasing evidence is now available showing that CD1-restricted T cell responses against non-peptide mycobacterial antigens could play a role in the immune resistance against tuberculosis. BCG, widely used in anti-tubercular vaccination, shares various constituents with Mycobacterium tuberculosis, but does not provide full protection. In the present study we have investigated the pattern of group 1 CD1 molecule expression in adherent mononuclear cells (AMNC) of human peripheral blood, infected in vitro with BCG. Shortly after exposure to BCG, both BCG-positive and BCG-negative AMNC showed a moderate CD1 expression elicited by BCG-induced release of GM-CSF presumably acting through an autocrine and a paracrine mechanism. This was demonstrated using two-color flow cytometry with green fluorescent BCG and anti-CD1 PE-labeled antibodies. However, high CD1 expression induced by exogenously added GM-CSF in AMNC was reduced if target cells were cocultivated with BCG. Monoclonal antibodies against IL-10 partially restored CD1 expression, thus showing that IL-10, released from infected AMNC, is involved, at least in part, in CD1 negative modulation. Therefore, through a complex cytokine network, including not yet identified factor(s), BCG triggers but does not allow full expression of CD1 on AMNC. It cannot be excluded that this mechanism could play a role in the limited efficiency of BCG vaccination.

Original languageEnglish
Pages (from-to)321-332
Number of pages12
JournalInternational Immunopharmacology
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Mycobacterium bovis
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-10
Vaccination
Mycobacterium tuberculosis
Flow Cytometry
Tuberculosis
Color
Monoclonal Antibodies
T-Lymphocytes
Antigens
Antibodies

Keywords

  • BCG
  • CD1
  • GM-CSF
  • IL-10
  • Monocytes

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

BCG-infected adherent mononuclear cells release cytokines that regulate group 1 CD1 molecule expression. / Prete, Salvatore Pasquale; Giuliani, Anna; D'Atri, Stefania; Graziani, Grazia; Balduzzi, Alessandra; Oggioni, Marco Rinaldo; Iona, Elisabetta; Girolomoni, Giampiero; Bonmassar, Laura; Romani, Luigina; Franzese, Ornella.

In: International Immunopharmacology, Vol. 7, No. 3, 03.2007, p. 321-332.

Research output: Contribution to journalArticle

Prete, SP, Giuliani, A, D'Atri, S, Graziani, G, Balduzzi, A, Oggioni, MR, Iona, E, Girolomoni, G, Bonmassar, L, Romani, L & Franzese, O 2007, 'BCG-infected adherent mononuclear cells release cytokines that regulate group 1 CD1 molecule expression', International Immunopharmacology, vol. 7, no. 3, pp. 321-332. https://doi.org/10.1016/j.intimp.2006.11.003
Prete, Salvatore Pasquale ; Giuliani, Anna ; D'Atri, Stefania ; Graziani, Grazia ; Balduzzi, Alessandra ; Oggioni, Marco Rinaldo ; Iona, Elisabetta ; Girolomoni, Giampiero ; Bonmassar, Laura ; Romani, Luigina ; Franzese, Ornella. / BCG-infected adherent mononuclear cells release cytokines that regulate group 1 CD1 molecule expression. In: International Immunopharmacology. 2007 ; Vol. 7, No. 3. pp. 321-332.
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