Bcl-2 immunoreactivity in salivary gland neoplasms is unrelated to the expression of mRNA for natural killer cell stimulatory cytokines interleukin (IL)-2 and IL-12

H. B. Hellquist, M. G. Karlsson, G. Viale, C. Karlsson, Å Davidsson, P. Dell'Orto, J. Olofsson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Certain cytokines are involved in the generation of natural killer (NK) cells and participate in the regulation of the proto-oncogene bcl-2. We aimed to study the mRNA expression of interleukin (IL) -2, IL-4 and IL-5, the composition of the tumour infiltrating lymphocytes (TIL), and the expression of bcl-2 in 14 benign and malignant human parotid tumours. TIL were predominantly composed of T lymphocytes and NK cells. We found evidence for the homing of T cells, and for generation of NK cells in the vicinity of the tumours, mRNA for IL-2 and IL-12, were identified but IL-4 mRNA was not found. The cytokine profiles and the composition of TIL of the two tumour categories were indistinguishable, suggesting that these host-response variables do not explain the differences in biological behaviour of these particular tumours. The results support a shift towards Th1 (T helper 1) cells and interferon-γ production, and that IL-12 also in vivo may play an important role in the regulatory interaction between innate resistance and adaptive immunity in tumour diseases. Most infiltrating lymphocytes showed strong expression of bcl-2; an interesting observation with regard to lymphocytic apoptosis in neoplastic diseases. The immunoreactivity fot the bcl-2 protein varied considerably between and within tumours, and almost all benign tumours showed strong bcl-2 positively whereas several of the malignant tumours showed weak or absent staining. The variable expression of bcl-2 protein suggests a different susceptibility of tumour cells to apoptosis. The results also indicate that bcl-2 cannot play a major role as protective agent in the specific apoptotic pathway induced by NK cells.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalVirchows Archiv
Volume429
Issue number2-3
Publication statusPublished - 1996

Fingerprint

Salivary Gland Neoplasms
Interleukin-12
Natural Killer Cells
Interleukin-2
Cytokines
Messenger RNA
Tumor-Infiltrating Lymphocytes
Neoplasms
Interleukin-4
Apoptosis
T-Lymphocytes
Protective Agents
Th1 Cells
Proto-Oncogenes
Interleukin-5
Adaptive Immunity
Innate Immunity
Interferons
Proteins
Observation

Keywords

  • Apoptosis
  • bcl-2
  • Cytokines
  • NK cells
  • Salivary gland neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bcl-2 immunoreactivity in salivary gland neoplasms is unrelated to the expression of mRNA for natural killer cell stimulatory cytokines interleukin (IL)-2 and IL-12. / Hellquist, H. B.; Karlsson, M. G.; Viale, G.; Karlsson, C.; Davidsson, Å; Dell'Orto, P.; Olofsson, J.

In: Virchows Archiv, Vol. 429, No. 2-3, 1996, p. 149-158.

Research output: Contribution to journalArticle

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abstract = "Certain cytokines are involved in the generation of natural killer (NK) cells and participate in the regulation of the proto-oncogene bcl-2. We aimed to study the mRNA expression of interleukin (IL) -2, IL-4 and IL-5, the composition of the tumour infiltrating lymphocytes (TIL), and the expression of bcl-2 in 14 benign and malignant human parotid tumours. TIL were predominantly composed of T lymphocytes and NK cells. We found evidence for the homing of T cells, and for generation of NK cells in the vicinity of the tumours, mRNA for IL-2 and IL-12, were identified but IL-4 mRNA was not found. The cytokine profiles and the composition of TIL of the two tumour categories were indistinguishable, suggesting that these host-response variables do not explain the differences in biological behaviour of these particular tumours. The results support a shift towards Th1 (T helper 1) cells and interferon-γ production, and that IL-12 also in vivo may play an important role in the regulatory interaction between innate resistance and adaptive immunity in tumour diseases. Most infiltrating lymphocytes showed strong expression of bcl-2; an interesting observation with regard to lymphocytic apoptosis in neoplastic diseases. The immunoreactivity fot the bcl-2 protein varied considerably between and within tumours, and almost all benign tumours showed strong bcl-2 positively whereas several of the malignant tumours showed weak or absent staining. The variable expression of bcl-2 protein suggests a different susceptibility of tumour cells to apoptosis. The results also indicate that bcl-2 cannot play a major role as protective agent in the specific apoptotic pathway induced by NK cells.",
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AU - Hellquist, H. B.

AU - Karlsson, M. G.

AU - Viale, G.

AU - Karlsson, C.

AU - Davidsson, Å

AU - Dell'Orto, P.

AU - Olofsson, J.

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