Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics

Research output: Contribution to journalArticle

Abstract

Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalArchives of Virology
Volume151
Issue number2
DOIs
Publication statusPublished - Feb 2006

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SARS Virus
Caspase 2
Virus Replication
Apoptosis
Vero Cells
Infection
Plasmids

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

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title = "Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics",
abstract = "Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells.",
author = "L. Bordi and C. Castilletti and L. Falasca and F. Ciccosanti and S. Calcaterra and G. Rozera and {Di Caro}, A. and S. Zaniratti and A. Rinaldi and G. Ippolito and M. Piacentini and Capobianchi, {M. R.}",
year = "2006",
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language = "English",
volume = "151",
pages = "369--377",
journal = "Archives of Virology",
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T1 - Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics

AU - Bordi, L.

AU - Castilletti, C.

AU - Falasca, L.

AU - Ciccosanti, F.

AU - Calcaterra, S.

AU - Rozera, G.

AU - Di Caro, A.

AU - Zaniratti, S.

AU - Rinaldi, A.

AU - Ippolito, G.

AU - Piacentini, M.

AU - Capobianchi, M. R.

PY - 2006/2

Y1 - 2006/2

N2 - Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells.

AB - Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells.

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JO - Archives of Virology

JF - Archives of Virology

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