Bcl-2 overexpression decreases BCNU sensitivity of a human glioblastoma line through enhancement of catalase activity

Donatella Del Bufalo, Daniela Trisciuoglio, Annamaria Biroccio, Lucia Marcocci, Simonetta Buglioni, Antonio Candiloro, Marco Scarsella, Carlo Leonetti, Gabriella Zupi

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate the role of bcl-2 in 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) sensitivity of the ADFS human glioblastoma cell line in vitro and in vivo. To this end, the ADFS line expressing a low level of the bcl-2 protein was transfected with a bcl-2 expression vector. We found that bcl-2 overexpressing clones were less sensitive to in vitro BCNU treatment than the control clone. Cell cycle analysis demonstrated that while BCNU induced a consistent block in S/G2-M phases of the cell cycle in the control clone, it did not affect the cell cycle phase distribution of the two bcl-2 transfectants. The different sensitivity to BCNU was unrelated to the ability of bcl-2 to inhibit apoptosis, while bcl-2 appeared to protect bcl-2 transfectants from BCNU toxicity through an increase of catalase activity. The ability of the catalase inhibitor, sodium azide, to increase the BCNU sensitivity of the bcl-2 transfectants to levels of the BCNU-treated control clone substantiated the role of the catalase activity. The effect of bcl-2 in reducing sensitivity to BCNU was also confirmed by in vivo experiments. Xenografts of bcl-2 overexpressing tumors were less sensitive to BCNU treatment than xenografts originating from control cells.

Original languageEnglish
Pages (from-to)473-483
Number of pages11
JournalJournal of Cellular Biochemistry
Volume83
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Bcl-2
  • BCNU
  • Catalase
  • Glioblastoma

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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