Bcl-2 protein is required for the adenine/uridine-rich element (ARE)-dependent degradation of its own messenger

Annamaria Bevilacqua, Maria Cristina Ceriani, Gianfranco Canti, Laura Asnaghi, Roberto Gherzi, Gary Brewer, Laura Papucci, Nicola Schiavone, Sergio Capaccioli, Angelo Nicolin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have shown previously that the decay of human bcl-2 mRNA is mediated by an adenine/uridine-rich element (ARE) located in the 3′-untranslated region. Here, we have utilized a non-radioactive cell-free mRNA decay system to investigate the biochemical and functional mechanisms regulating the ARE-dependent degradation of bcl-2 mRNA. Using RNA substrates, mutants, and competitors, we found that decay is specific and ARE-dependent, although maximized by the ARE-flanking regions. In unfractionated extracts from different cell types and in whole cells, the relative enzymatic activity was related to the amount of Bcl-2 protein expressed by the cells at steady state. The degradation activity was lost upon Bcl-2 depletion and was reconstituted by adding recombinant Bcl-2. Ineffective extracts from cells that constitutively do not express Bcl-2 acquire full degradation activity by adding recombinant Bcl-2 protein. We conclude that Bcl-2 is necessary to activate the degradation complex on the relevant RNA target.

Original languageEnglish
Pages (from-to)23451-23459
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number26
DOIs
Publication statusPublished - Jul 27 2003

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Uridine
Adenine
Degradation
Cell Extracts
Messenger RNA
Proteins
RNA
RNA Stability
3' Untranslated Regions
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bcl-2 protein is required for the adenine/uridine-rich element (ARE)-dependent degradation of its own messenger. / Bevilacqua, Annamaria; Ceriani, Maria Cristina; Canti, Gianfranco; Asnaghi, Laura; Gherzi, Roberto; Brewer, Gary; Papucci, Laura; Schiavone, Nicola; Capaccioli, Sergio; Nicolin, Angelo.

In: Journal of Biological Chemistry, Vol. 278, No. 26, 27.07.2003, p. 23451-23459.

Research output: Contribution to journalArticle

Bevilacqua, A, Ceriani, MC, Canti, G, Asnaghi, L, Gherzi, R, Brewer, G, Papucci, L, Schiavone, N, Capaccioli, S & Nicolin, A 2003, 'Bcl-2 protein is required for the adenine/uridine-rich element (ARE)-dependent degradation of its own messenger', Journal of Biological Chemistry, vol. 278, no. 26, pp. 23451-23459. https://doi.org/10.1074/jbc.M210620200
Bevilacqua, Annamaria ; Ceriani, Maria Cristina ; Canti, Gianfranco ; Asnaghi, Laura ; Gherzi, Roberto ; Brewer, Gary ; Papucci, Laura ; Schiavone, Nicola ; Capaccioli, Sergio ; Nicolin, Angelo. / Bcl-2 protein is required for the adenine/uridine-rich element (ARE)-dependent degradation of its own messenger. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 26. pp. 23451-23459.
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AU - Asnaghi, Laura

AU - Gherzi, Roberto

AU - Brewer, Gary

AU - Papucci, Laura

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