Bcl-6 protein expression, and not the germinal centre immunophenotype, predicts favourable prognosis in a series of primary nodal diffuse large B-cell lymphomas: A single centre experience

Silvia Uccella, Claudia Placidi, Silvia Marchet, Massimiliano Cergnul, Ilaria Proserpio, Claudio Chini, Raffaele Novario, Graziella Pinotti, Carlo Capella

Research output: Contribution to journalArticlepeer-review

Abstract

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease. The immunohistochemistry-based algorithms for the determination of the cell of origin of DLBCL have been proposed as a practical method to validate and surrogate results obtained by gene expression profiling. We studied 71 patients with primary nodal DLBCL at diagnosis, who received anthracycline-based therapy with or without rituximab. Immunohistochemistry was performed using anti-CD10, Bcl-6, MUM1 and Bcl-2 antibodies in order to assess the ontogenic profile of neoplastic cells and to verify its relation with clinical outcome. Survival data were analysed using an explorative Cox model. The immunohistochemistry-based algorithms for the determination of the cell of origin of DLBCL were not associated with prognosis. By contrast, Bcl-6 expression was associated with a longer lymphoma-free survival while immunoreactivities for MUM1 or Bcl-2 were not significantly related to patient outcome. Bcl-6 expression alone proved to be a prognostic marker in primary nodal DLBCL and seemed to be more reliable to predict clinical outcome in these disorders than the immunohistochemical algorithms for the detection of the germinal centre/non-germinal centre immunophenotype.

Original languageEnglish
Pages (from-to)1321-1328
Number of pages8
JournalLeukemia and Lymphoma
Volume49
Issue number7
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Bcl-6
  • CD10
  • Diffuse large B-cell lymphoma
  • Germinal centre phenotype
  • Immunohistochemistry

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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