BCL-6 protein expression in AIDS-related non-Hodgkin's lymphomas: Inverse relationship with Epstein-Barr virus-encoded latent membrane protein- 1 expression

Antonino Carbone, Gianluca Gaidano, Annunziata Gloghini, Cristina Pastore, Giuseppe Saglio, Umberto Tirelli, Riccardo Dalla-Favera, Brunangelo Falini

Research output: Contribution to journalArticle

Abstract

This study aimed at investigating the expression of the BCL-6 protein in acquired immune deficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (AIDS-NHLs) and at comparing the expression pattern with the presence of Epstein-Barr virus (EBV) infection of the tumor clone. A total of 34 AIDS- NHL biopsies, including 16 AIDS-related diffuse large-cell lymphomas (AIDS- DLCLs) and 18 AIDS-related small-noncleaved-cell lymphomas (AIDS-SNCCLs) as well as 7 AIDS-SNCCL cell lines were immunostained with a BCL-6-specific monoclonal antibody. Expression of BCL-6 protein was detected in 9 of 16 AIDS-DLCLs, 18 of 18 AIDS-SNCCLs, and 3 of 7 AIDS- SNCCL cell lines. Expression of BCL-6 among AIDS-NHLs occurred independently of the presence of molecular lesions of the bcl-6 gene. Notably, among EBV-positive AIDS-NHL biopsies and cell lines, expression of BCL-6 was mutually exclusive with the expression of EBV-encoded transforming antigen latent membrane protein-1. The mutual exclusion between BCL-6 and latent membrane protein-1 expression was further confirmed by in vitro superinfection experiments of an AIDS-SNCCL cell line originally devoid of EBV sequences. Overall, the frequent association between AIDS-NHLs and expression of BCL-6, a protein selectively expressed by germinal center B cells, suggests that these lymphomas may originate from the germinal center.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalAmerican Journal of Pathology
Volume150
Issue number1
Publication statusPublished - 1997

Fingerprint

Non-Hodgkin's Lymphoma
Acquired Immunodeficiency Syndrome
Proteins
Human Herpesvirus 4
Cell Line
Germinal Center
Epstein-Barr virus EBV-associated membrane antigen
Membrane Proteins
Biopsy
Superinfection
Epstein-Barr Virus Infections
Lymphoma, Large B-Cell, Diffuse
Viral Tumor Antigens
B-Cell Lymphoma
Clone Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

BCL-6 protein expression in AIDS-related non-Hodgkin's lymphomas : Inverse relationship with Epstein-Barr virus-encoded latent membrane protein- 1 expression. / Carbone, Antonino; Gaidano, Gianluca; Gloghini, Annunziata; Pastore, Cristina; Saglio, Giuseppe; Tirelli, Umberto; Dalla-Favera, Riccardo; Falini, Brunangelo.

In: American Journal of Pathology, Vol. 150, No. 1, 1997, p. 155-165.

Research output: Contribution to journalArticle

Carbone, Antonino ; Gaidano, Gianluca ; Gloghini, Annunziata ; Pastore, Cristina ; Saglio, Giuseppe ; Tirelli, Umberto ; Dalla-Favera, Riccardo ; Falini, Brunangelo. / BCL-6 protein expression in AIDS-related non-Hodgkin's lymphomas : Inverse relationship with Epstein-Barr virus-encoded latent membrane protein- 1 expression. In: American Journal of Pathology. 1997 ; Vol. 150, No. 1. pp. 155-165.
@article{d02074e633a94253be481b9fb1844e7a,
title = "BCL-6 protein expression in AIDS-related non-Hodgkin's lymphomas: Inverse relationship with Epstein-Barr virus-encoded latent membrane protein- 1 expression",
abstract = "This study aimed at investigating the expression of the BCL-6 protein in acquired immune deficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (AIDS-NHLs) and at comparing the expression pattern with the presence of Epstein-Barr virus (EBV) infection of the tumor clone. A total of 34 AIDS- NHL biopsies, including 16 AIDS-related diffuse large-cell lymphomas (AIDS- DLCLs) and 18 AIDS-related small-noncleaved-cell lymphomas (AIDS-SNCCLs) as well as 7 AIDS-SNCCL cell lines were immunostained with a BCL-6-specific monoclonal antibody. Expression of BCL-6 protein was detected in 9 of 16 AIDS-DLCLs, 18 of 18 AIDS-SNCCLs, and 3 of 7 AIDS- SNCCL cell lines. Expression of BCL-6 among AIDS-NHLs occurred independently of the presence of molecular lesions of the bcl-6 gene. Notably, among EBV-positive AIDS-NHL biopsies and cell lines, expression of BCL-6 was mutually exclusive with the expression of EBV-encoded transforming antigen latent membrane protein-1. The mutual exclusion between BCL-6 and latent membrane protein-1 expression was further confirmed by in vitro superinfection experiments of an AIDS-SNCCL cell line originally devoid of EBV sequences. Overall, the frequent association between AIDS-NHLs and expression of BCL-6, a protein selectively expressed by germinal center B cells, suggests that these lymphomas may originate from the germinal center.",
author = "Antonino Carbone and Gianluca Gaidano and Annunziata Gloghini and Cristina Pastore and Giuseppe Saglio and Umberto Tirelli and Riccardo Dalla-Favera and Brunangelo Falini",
year = "1997",
language = "English",
volume = "150",
pages = "155--165",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - BCL-6 protein expression in AIDS-related non-Hodgkin's lymphomas

T2 - Inverse relationship with Epstein-Barr virus-encoded latent membrane protein- 1 expression

AU - Carbone, Antonino

AU - Gaidano, Gianluca

AU - Gloghini, Annunziata

AU - Pastore, Cristina

AU - Saglio, Giuseppe

AU - Tirelli, Umberto

AU - Dalla-Favera, Riccardo

AU - Falini, Brunangelo

PY - 1997

Y1 - 1997

N2 - This study aimed at investigating the expression of the BCL-6 protein in acquired immune deficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (AIDS-NHLs) and at comparing the expression pattern with the presence of Epstein-Barr virus (EBV) infection of the tumor clone. A total of 34 AIDS- NHL biopsies, including 16 AIDS-related diffuse large-cell lymphomas (AIDS- DLCLs) and 18 AIDS-related small-noncleaved-cell lymphomas (AIDS-SNCCLs) as well as 7 AIDS-SNCCL cell lines were immunostained with a BCL-6-specific monoclonal antibody. Expression of BCL-6 protein was detected in 9 of 16 AIDS-DLCLs, 18 of 18 AIDS-SNCCLs, and 3 of 7 AIDS- SNCCL cell lines. Expression of BCL-6 among AIDS-NHLs occurred independently of the presence of molecular lesions of the bcl-6 gene. Notably, among EBV-positive AIDS-NHL biopsies and cell lines, expression of BCL-6 was mutually exclusive with the expression of EBV-encoded transforming antigen latent membrane protein-1. The mutual exclusion between BCL-6 and latent membrane protein-1 expression was further confirmed by in vitro superinfection experiments of an AIDS-SNCCL cell line originally devoid of EBV sequences. Overall, the frequent association between AIDS-NHLs and expression of BCL-6, a protein selectively expressed by germinal center B cells, suggests that these lymphomas may originate from the germinal center.

AB - This study aimed at investigating the expression of the BCL-6 protein in acquired immune deficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (AIDS-NHLs) and at comparing the expression pattern with the presence of Epstein-Barr virus (EBV) infection of the tumor clone. A total of 34 AIDS- NHL biopsies, including 16 AIDS-related diffuse large-cell lymphomas (AIDS- DLCLs) and 18 AIDS-related small-noncleaved-cell lymphomas (AIDS-SNCCLs) as well as 7 AIDS-SNCCL cell lines were immunostained with a BCL-6-specific monoclonal antibody. Expression of BCL-6 protein was detected in 9 of 16 AIDS-DLCLs, 18 of 18 AIDS-SNCCLs, and 3 of 7 AIDS- SNCCL cell lines. Expression of BCL-6 among AIDS-NHLs occurred independently of the presence of molecular lesions of the bcl-6 gene. Notably, among EBV-positive AIDS-NHL biopsies and cell lines, expression of BCL-6 was mutually exclusive with the expression of EBV-encoded transforming antigen latent membrane protein-1. The mutual exclusion between BCL-6 and latent membrane protein-1 expression was further confirmed by in vitro superinfection experiments of an AIDS-SNCCL cell line originally devoid of EBV sequences. Overall, the frequent association between AIDS-NHLs and expression of BCL-6, a protein selectively expressed by germinal center B cells, suggests that these lymphomas may originate from the germinal center.

UR - http://www.scopus.com/inward/record.url?scp=0031021935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031021935&partnerID=8YFLogxK

M3 - Article

C2 - 9006332

AN - SCOPUS:0031021935

VL - 150

SP - 155

EP - 165

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 1

ER -