Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells

Valérie Robert, Pamela Gurlini, Valeria Tosello, Takeharu Nagai, Atsushi Miyawaki, Fabio Di Lisa, Tullio Pozzan

Research output: Contribution to journalArticlepeer-review

Abstract

The Ca2+-sensitive photoprotein aequorin and the new green fluorescent protein-based fluorescent Ca2+ indicators 'ratiometric-pericam' were selectively expressed in the mitochondria, cytosol and/or nucleus of spontaneously beating ventricular myocytes from neonatal rats. This combined strategy reveals that mitochondrial [Ca2+] oscillates rapidly and in synchrony with cytosolic and nuclear [Ca2+]. The Ca2+ oscillations were reduced in frequency and/or amplitude by verapamil and carbachol and were enhanced by isoproterenol and elevation of extracellular [Ca2+]. An increased frequency and/or amplitude of cytosolic Ca2+ spikes was rapidly mirrored by similar changes in mitochondrial Ca2+ spikes and more slowly by elevations of the interspike Ca2+ levels. The present data unequivocally demonstrate that in cardiac cells mitochondrial [Ca2+] oscillates synchronously with cytosolic [Ca2+] and that mitochondrial Ca2+ handling rapidly adapts to inotropic or chronotropic inputs.

Original languageEnglish
Pages (from-to)4998-5007
Number of pages10
JournalEMBO Journal
Volume20
Issue number17
DOIs
Publication statusPublished - Sep 3 2001

Keywords

  • Aequorin
  • Contraction
  • Mitochondrial Ca
  • Neonatal cardiac cells
  • Pericam

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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