Behaviour of some lysosomal enzymes in the plasma of insulin dependent diabetic patients during artificial pancreas treatment

A. B. Burlina, G. Goi, A. Fabi, A. Lombardo, D. Gaburro, G. Tettamanti

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The plasma levels of three lysosomal enzymes, β-D-N-acetylglucosaminidase, β-D-glucuronidase, and α-L-fucosidase, were fluorimetrically determined in seven insulin-dependent diabetic patients one day before, one day after, and during a two-day treatment with the artificial pancreas, at 4 to 5 h intervals. A statistically significant decrease of the plasma level of each enzymes was observed during artificial pancreas treatment. The extent of decrease was 30 to 35% for β-N-acetylglucosaminidase, 35 to 40% for β-D-glucuronidase, and 20 to 25% for α-L-fucosidase. The decrease occurred earlier (at the first day of treatment) for β-D-N-acetylglucosaminidase, and later (at the second day of treatment, and lasting to the first day after treatment) for the other two enzymes. These results suggest a direct connection between the lysosomal apparatus and insulin-controlled metabolic pathways, and a potential role for lysosomal enzymes as indicators of the metabolic compensation in diabetes.

Original languageEnglish
Pages (from-to)423-427
Number of pages5
JournalClinical Biochemistry
Volume20
Issue number6
DOIs
Publication statusPublished - 1987

Fingerprint

Artificial Pancreas
Acetylglucosaminidase
alpha-L-Fucosidase
Glucuronidase
Insulin
4 alpha-glucanotransferase
Plasmas
Enzymes
Medical problems
Therapeutics
Metabolic Networks and Pathways

Keywords

  • artificial pancreas
  • diabetes
  • lysosomal enzymes
  • plasma clinical enzymology

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Behaviour of some lysosomal enzymes in the plasma of insulin dependent diabetic patients during artificial pancreas treatment. / Burlina, A. B.; Goi, G.; Fabi, A.; Lombardo, A.; Gaburro, D.; Tettamanti, G.

In: Clinical Biochemistry, Vol. 20, No. 6, 1987, p. 423-427.

Research output: Contribution to journalArticle

Burlina, A. B. ; Goi, G. ; Fabi, A. ; Lombardo, A. ; Gaburro, D. ; Tettamanti, G. / Behaviour of some lysosomal enzymes in the plasma of insulin dependent diabetic patients during artificial pancreas treatment. In: Clinical Biochemistry. 1987 ; Vol. 20, No. 6. pp. 423-427.
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AU - Fabi, A.

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AU - Gaburro, D.

AU - Tettamanti, G.

PY - 1987

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AB - The plasma levels of three lysosomal enzymes, β-D-N-acetylglucosaminidase, β-D-glucuronidase, and α-L-fucosidase, were fluorimetrically determined in seven insulin-dependent diabetic patients one day before, one day after, and during a two-day treatment with the artificial pancreas, at 4 to 5 h intervals. A statistically significant decrease of the plasma level of each enzymes was observed during artificial pancreas treatment. The extent of decrease was 30 to 35% for β-N-acetylglucosaminidase, 35 to 40% for β-D-glucuronidase, and 20 to 25% for α-L-fucosidase. The decrease occurred earlier (at the first day of treatment) for β-D-N-acetylglucosaminidase, and later (at the second day of treatment, and lasting to the first day after treatment) for the other two enzymes. These results suggest a direct connection between the lysosomal apparatus and insulin-controlled metabolic pathways, and a potential role for lysosomal enzymes as indicators of the metabolic compensation in diabetes.

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