Behavioural stress blunts the creatinine clearance increase induced by a protein load in healthy subjects

L. Centoza, G. Castoldi, G. Busca, A. Stella, A. Zanchetti, R. Golin

Research output: Contribution to journalArticlepeer-review


Background: The aim of this study was to investigate how behavioural states related to different levels of stress affected the increments of glomerular filtration rate induced by an acute protein load. Methods: Thirteen healthy subjects were enrolled. Each subject was studied from 9:00 h to 15:00 h on two consecutive days. In random order, after a protein meal (1.2 g/kg b.w. of protein), each subject was required to remain in a relaxing, sitting position (resting period, R), or to solve graphical and mathematical problems (behavioural stress period, S). Mean blood pressure (MBP) and heart rate (HR) were monitored by an ambulatory blood pressure device. Urine samples collected in each period were used to measure glomerular filtration rate (GFR, creatinine clearance) and urinary sodium excretion (UNa+V). Results: Significant decreases in MBP and HR were observed during the resting period after the protein load, which significantly increased GFR. There was also a large increase of UNa+V. During S, the GFR changes were no longer seen whereas the increase of UNa+V was maintained. HR and MBP did not change compared to the pre-stimulus period. Conclusions: We conclude that in healthy subjects behavioural stress can blunt the increase in GFR that follows a protein load, presumably by neuro-humoral activated mechanisms. In our expeerimental conditions, behavioural stress did not affect UNa+V.

Original languageEnglish
Pages (from-to)403-409
Number of pages7
JournalJournal of Nephrology
Issue number5
Publication statusPublished - 2001


  • Behavioural stress
  • Glomerular filtration rate
  • Healthy subjects
  • Protein load
  • Renal functional reserve

ASJC Scopus subject areas

  • Nephrology


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