Beneficial effects of a plant histaminase in a rat model of splanchnic artery occlusion and reperfusion

Emanuela Masini, Salvatore Cuzzocrea, Daniele Bani, Emanuela Mazzon, Carmelo Muja, Rosanna Mastroianni, Francesca Fabrizi, Paola Pietrangeli, Lucia Marcocci, Bruno Mondovì, Pier Francesco Mannaioni, Rodolfo Federico

Research output: Contribution to journalArticlepeer-review


Splanchnic artery occlusion (SAO) followed by reperfusion causes endothelial injury and inflammation which contribute to the pathophysiology of shock. We investigated the effects of pea seedling (Latyrus cicera) histaminase, known to afford protection against the deleterious effects of cardiac ischemia/reperfusion, given to rats subjected to SAO/reperfusion-induced splanchnic injury. Histaminase (80 IU kg, 15 min before reperfusion) significantly reduced the drop of blood pressure and high mortality rate caused by SAO/reperfusion. Histaminase also reduced histopathological changes, leukocyte infiltration (myeloperoxidase), and expression of endothelial cell adhesion molecules in the ileum. Histaminase counteracted free radical-mediated tissue injury, as judged by a significant decrease in the plasma and tissue levels of peroxidation and nitration products (oxidized rhodamine, malondialdehyde, nitrotyrosine), DNA damage markers (8-hydroxy-2-deoxyguanosine, poly-adenosine diphosphate-ribosylated DNA) and consumption of tissue antioxidant enzymes (superoxide dismutase). As a result, histaminase led to a reduction of ileal cell apoptosis (caspase 3, terminal deoxynucleotidyltransferase-mediated UTP end labeling-positive cells). These results show that histaminase exerts a clear-cut protective effect in SAO/reperfusion-induced splanchnic injury, likely caused by oxidative catabolism of proinflammatory histamine and antioxidant effects resulting in hindrance of free radical-mediated tissue injury, endothelial dysfunction, and leukocyte recruitment. Thus, histaminase could be used therapeutically in intestinal ischemia.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
Issue number4
Publication statusPublished - Apr 2007


  • Apoptosis
  • Endothelial cell adhesion molecules
  • Histaminase
  • Oxygen free radicals
  • Splanchnic artery occlusion

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology


Dive into the research topics of 'Beneficial effects of a plant histaminase in a rat model of splanchnic artery occlusion and reperfusion'. Together they form a unique fingerprint.

Cite this