Beneficial effects of tempol, a membrane-permeable radical scavenger, in a rodent model of splanchnic artery occlusion and reperfusion

Salvatore Cuzzocrea, Michelle C. McDonald, Emanuela Mazzon, Helder Mota Filipe, Giuseppina Costantino, Achille P. Caputi, Christoph Thiemermann

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present study was to investigate the effects of tempol, a membrane-permeable radical scavenger, in rats subjected to splanchnic artery occlusion shock (SAO). Rats subjected to SAO developed a significant decrease in mean arterial blood pressure, a significant increase in tissue myeloperoxidase activity, and a marked injury to the distal ileum. SAO shock resulted in 100% mortality at 2 h after reperfusion. At 60 min after reperfusion, a marked increase in the immunoreactivity to nitrotyrosine and to poly (ADP-ribose) synthetase was observed in the necrotic ileum of rats with SAO. Staining of sections of the ileum obtained from SAO-shocked rats with anti-intercellular adhesion molecule (ICAM-1) and anti-P-selectin antibodies resulted in diffuse staining. Tempol (30 mg/kg bolus injection 5 min prior to reperfusion, followed by an infusion of 30 mg/kg/h intravenously) attenuated 1) the infiltration of the reperfused intestine with neutrophils, 2) the lipid peroxidation, 3) the production of peroxynitrite, 4) the degree of P-selectin and ICAM-1 staining in tissue sections from SAO-shocked rats, 5) histological signs of bowel injury, and 6) mortality at 2 h after reperfusion. Taken together, our results clearly demonstrate that the intracellular radical scavenger tempol reduces the intestinal injury of rats subjected SAO shock.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalShock
Volume14
Issue number2
Publication statusPublished - Aug 2000

Keywords

  • Adhesion molecule
  • Free radicals
  • ICAM-1
  • Lipid peroxidation
  • P-selectin
  • Peroxynitrite
  • Splanchnic artery occlusion (SAO) shock
  • Tempol

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

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