Benzene adducts with rat nucleic acids and proteins

Dose-response relationship after treatment in vivo

M. Mazzullo, S. Bartoli, B. Bonora, A. Colacci, S. Grilli, G. Lattanzi, A. Niero, M. P. Turina, S. Parodi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The dose-response relationship of the benzene covalent interaction with biological macromolecules from rat organs was studied. The administered dose range was 3.6 x 107 starting from the highest dosage employed, 486 mg/kg, which is oncogenic for rodents, and included low and very low dosages. The present study was initially performed with tritum-labeled benzene, administered by IP injection. In order to exclude the possibility that part of the detected radioactivity was due to tritium incorporated into DNA from metabolic processes, 14C-benzene was then also used following a similar experimental design. By HPLC analysis, a single adduct from benzene-treated DNA was detected; adduct identification will be attempted in the near future. Linear dose-response relationship was observed within most of the range of explored doses. Linearity was particularly evident within low and very low dosages. Saturation of benzene metabolism did occur at the highest dosages for most of the assayed macromolecules and organs, especially in rat liver. This finding could be considered as indicative of the dose-response relationship of tumor induction and could be used in risk assessment.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalEnvironmental Health Perspectives
Volume82
Publication statusPublished - 1989

Fingerprint

dose-response relationship
nucleic acid
Benzene
Nucleic Acids
benzene
Rats
protein
Proteins
Macromolecules
Therapeutics
DNA
Tritium
Radioactivity
tritium
linearity
radioactivity
Metabolism
tumor
Liver
Risk assessment

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Mazzullo, M., Bartoli, S., Bonora, B., Colacci, A., Grilli, S., Lattanzi, G., ... Parodi, S. (1989). Benzene adducts with rat nucleic acids and proteins: Dose-response relationship after treatment in vivo. Environmental Health Perspectives, 82, 259-266.

Benzene adducts with rat nucleic acids and proteins : Dose-response relationship after treatment in vivo. / Mazzullo, M.; Bartoli, S.; Bonora, B.; Colacci, A.; Grilli, S.; Lattanzi, G.; Niero, A.; Turina, M. P.; Parodi, S.

In: Environmental Health Perspectives, Vol. 82, 1989, p. 259-266.

Research output: Contribution to journalArticle

Mazzullo, M, Bartoli, S, Bonora, B, Colacci, A, Grilli, S, Lattanzi, G, Niero, A, Turina, MP & Parodi, S 1989, 'Benzene adducts with rat nucleic acids and proteins: Dose-response relationship after treatment in vivo', Environmental Health Perspectives, vol. 82, pp. 259-266.
Mazzullo, M. ; Bartoli, S. ; Bonora, B. ; Colacci, A. ; Grilli, S. ; Lattanzi, G. ; Niero, A. ; Turina, M. P. ; Parodi, S. / Benzene adducts with rat nucleic acids and proteins : Dose-response relationship after treatment in vivo. In: Environmental Health Perspectives. 1989 ; Vol. 82. pp. 259-266.
@article{7838a95c72b94ea6bbc221a9e28ed57a,
title = "Benzene adducts with rat nucleic acids and proteins: Dose-response relationship after treatment in vivo",
abstract = "The dose-response relationship of the benzene covalent interaction with biological macromolecules from rat organs was studied. The administered dose range was 3.6 x 107 starting from the highest dosage employed, 486 mg/kg, which is oncogenic for rodents, and included low and very low dosages. The present study was initially performed with tritum-labeled benzene, administered by IP injection. In order to exclude the possibility that part of the detected radioactivity was due to tritium incorporated into DNA from metabolic processes, 14C-benzene was then also used following a similar experimental design. By HPLC analysis, a single adduct from benzene-treated DNA was detected; adduct identification will be attempted in the near future. Linear dose-response relationship was observed within most of the range of explored doses. Linearity was particularly evident within low and very low dosages. Saturation of benzene metabolism did occur at the highest dosages for most of the assayed macromolecules and organs, especially in rat liver. This finding could be considered as indicative of the dose-response relationship of tumor induction and could be used in risk assessment.",
author = "M. Mazzullo and S. Bartoli and B. Bonora and A. Colacci and S. Grilli and G. Lattanzi and A. Niero and Turina, {M. P.} and S. Parodi",
year = "1989",
language = "English",
volume = "82",
pages = "259--266",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",

}

TY - JOUR

T1 - Benzene adducts with rat nucleic acids and proteins

T2 - Dose-response relationship after treatment in vivo

AU - Mazzullo, M.

AU - Bartoli, S.

AU - Bonora, B.

AU - Colacci, A.

AU - Grilli, S.

AU - Lattanzi, G.

AU - Niero, A.

AU - Turina, M. P.

AU - Parodi, S.

PY - 1989

Y1 - 1989

N2 - The dose-response relationship of the benzene covalent interaction with biological macromolecules from rat organs was studied. The administered dose range was 3.6 x 107 starting from the highest dosage employed, 486 mg/kg, which is oncogenic for rodents, and included low and very low dosages. The present study was initially performed with tritum-labeled benzene, administered by IP injection. In order to exclude the possibility that part of the detected radioactivity was due to tritium incorporated into DNA from metabolic processes, 14C-benzene was then also used following a similar experimental design. By HPLC analysis, a single adduct from benzene-treated DNA was detected; adduct identification will be attempted in the near future. Linear dose-response relationship was observed within most of the range of explored doses. Linearity was particularly evident within low and very low dosages. Saturation of benzene metabolism did occur at the highest dosages for most of the assayed macromolecules and organs, especially in rat liver. This finding could be considered as indicative of the dose-response relationship of tumor induction and could be used in risk assessment.

AB - The dose-response relationship of the benzene covalent interaction with biological macromolecules from rat organs was studied. The administered dose range was 3.6 x 107 starting from the highest dosage employed, 486 mg/kg, which is oncogenic for rodents, and included low and very low dosages. The present study was initially performed with tritum-labeled benzene, administered by IP injection. In order to exclude the possibility that part of the detected radioactivity was due to tritium incorporated into DNA from metabolic processes, 14C-benzene was then also used following a similar experimental design. By HPLC analysis, a single adduct from benzene-treated DNA was detected; adduct identification will be attempted in the near future. Linear dose-response relationship was observed within most of the range of explored doses. Linearity was particularly evident within low and very low dosages. Saturation of benzene metabolism did occur at the highest dosages for most of the assayed macromolecules and organs, especially in rat liver. This finding could be considered as indicative of the dose-response relationship of tumor induction and could be used in risk assessment.

UR - http://www.scopus.com/inward/record.url?scp=0024425559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024425559&partnerID=8YFLogxK

M3 - Article

VL - 82

SP - 259

EP - 266

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

ER -