Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells

David Drutovic, Martina Chripkova, Martina Pilatova, Peter Kruzliak, Pal Perjesi, Marek Sarissky, Monica Lupi, Giovanna Damia, Massimo Broggini, Jan Mojzis

Research output: Contribution to journalArticle

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Abstract

Colorectal cancer is the third most common cancer in the world, with 1.2 million new cancer cases annually. Chalcones are secondary metabolite precursors of flavonoids that exhibit diverse biological activities, including antioxidant and antitumor activities. The aim of this study was to investigate the antiproliferative effect of new synthetic chalcone derivatives on HCT116 cells. (E)-2-(2′,4′-dimethoxybenzylidene)-1- tetralone (Q705) was found to be the most active (IC50=3.44 ±0.25 μM). Based on these results, this compound was chosen for further analysis of its biochemical and molecular mechanisms. Our results showed that Q705 inhibited the growth and clonogenicity of HCT116 cells. The results of a flow cytometric analyses suggested that this compound caused a significant cell cycle arrest in G2/M phase and increased the proportion of cells in the subG0/G1 phase, marker of apoptosis. Q705-induced apoptosis was confirmed by TdT-mediated dUTP nick end labelling (TUNEL) assay. Treatment of HCT116 cells with this chalcone significantly increased the caspase-3,-7 activity and resulted in cleavage of poly-ADP-ribose polymerase (PARP). Changes in the nuclear morphology such as chromatin condensation were also observed. These effects were associated with a decreased expression of bcl-xL and increased overall ratio of bax/bcl-xL mRNA levels. Immunofluorescence and qRT-PCR analysis revealed that Q705 induced H2AX histone modifications characteristic of DNA damage, disruption of microtubule organization and downregulation of tubulins. In summary, these results suggest that the cyclic chalcone analogue Q705 has potential as a new compound for colorectal cancer therapy.

Original languageEnglish
Pages (from-to)9967-9975
Number of pages9
JournalTumor Biology
Volume35
Issue number10
DOIs
Publication statusPublished - 2014

Fingerprint

HCT116 Cells
Chalcone
Cell Cycle Checkpoints
Colorectal Neoplasms
Apoptosis
Histone Code
Tetralones
Chalcones
Caspase 7
Poly(ADP-ribose) Polymerases
G2 Phase
G1 Phase
Tubulin
Flavonoids
Microtubules
Caspase 3
Cell Division
Inhibitory Concentration 50
DNA Damage
Chromatin

Keywords

  • Apoptosis
  • Benzylidenetetralones
  • Cell cycle arrest
  • Colorectal cancer cells
  • Cyclic chalcone analogues

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)

Cite this

Drutovic, D., Chripkova, M., Pilatova, M., Kruzliak, P., Perjesi, P., Sarissky, M., ... Mojzis, J. (2014). Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells. Tumor Biology, 35(10), 9967-9975. https://doi.org/10.1007/s13277-014-2289-y

Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells. / Drutovic, David; Chripkova, Martina; Pilatova, Martina; Kruzliak, Peter; Perjesi, Pal; Sarissky, Marek; Lupi, Monica; Damia, Giovanna; Broggini, Massimo; Mojzis, Jan.

In: Tumor Biology, Vol. 35, No. 10, 2014, p. 9967-9975.

Research output: Contribution to journalArticle

Drutovic, David ; Chripkova, Martina ; Pilatova, Martina ; Kruzliak, Peter ; Perjesi, Pal ; Sarissky, Marek ; Lupi, Monica ; Damia, Giovanna ; Broggini, Massimo ; Mojzis, Jan. / Benzylidenetetralones, cyclic chalcone analogues, induce cell cycle arrest and apoptosis in HCT116 colorectal cancer cells. In: Tumor Biology. 2014 ; Vol. 35, No. 10. pp. 9967-9975.
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AU - Kruzliak, Peter

AU - Perjesi, Pal

AU - Sarissky, Marek

AU - Lupi, Monica

AU - Damia, Giovanna

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AU - Mojzis, Jan

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