Ber-H2 (anti-CD30)-saporin immunotoxin: A new tool for the treatment of Hodgkin's disease and CD30+ lymphoma: In vitro evaluation

P. L. Tazzari, A. Bolognesi, D. De Totero, B. Falini, R. M. Lemoli, M. R. Soria, S. Pileri, M. Gobbi, H. Stein, L. Flenghi, M. F. Martelli, F. Stirpe

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Abstract

An immunotoxin containing an anti-CD30 monoclonal antibody (Ber-H2) and saporin, a ribosome-inactivating protein type 1, is described. It specifically inhibits protein synthesis by Hodgkin derived target cell lines with a very high efficiency (IC50 ranging from 5 x 10-12 M to 5 x 10-14 M, as saporin), while irrelevant immunotoxins do not. Present results suggest that this immunotoxin could be used for in vivo therapy as well as for ex vivo bone marrow purging in Hodgkin's disease and CD30+ lymphomas.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalBritish Journal of Haematology
Volume81
Issue number2
Publication statusPublished - 1992

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Immunotoxins
Hodgkin Disease
Lymphoma
Type 1 Ribosome Inactivating Proteins
Bone Marrow Purging
Inhibitory Concentration 50
Therapeutics
Monoclonal Antibodies
Cell Line
In Vitro Techniques
saporin
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Ber-H2 (anti-CD30)-saporin immunotoxin : A new tool for the treatment of Hodgkin's disease and CD30+ lymphoma: In vitro evaluation. / Tazzari, P. L.; Bolognesi, A.; De Totero, D.; Falini, B.; Lemoli, R. M.; Soria, M. R.; Pileri, S.; Gobbi, M.; Stein, H.; Flenghi, L.; Martelli, M. F.; Stirpe, F.

In: British Journal of Haematology, Vol. 81, No. 2, 1992, p. 203-211.

Research output: Contribution to journalArticle

Tazzari, PL, Bolognesi, A, De Totero, D, Falini, B, Lemoli, RM, Soria, MR, Pileri, S, Gobbi, M, Stein, H, Flenghi, L, Martelli, MF & Stirpe, F 1992, 'Ber-H2 (anti-CD30)-saporin immunotoxin: A new tool for the treatment of Hodgkin's disease and CD30+ lymphoma: In vitro evaluation', British Journal of Haematology, vol. 81, no. 2, pp. 203-211.
Tazzari, P. L. ; Bolognesi, A. ; De Totero, D. ; Falini, B. ; Lemoli, R. M. ; Soria, M. R. ; Pileri, S. ; Gobbi, M. ; Stein, H. ; Flenghi, L. ; Martelli, M. F. ; Stirpe, F. / Ber-H2 (anti-CD30)-saporin immunotoxin : A new tool for the treatment of Hodgkin's disease and CD30+ lymphoma: In vitro evaluation. In: British Journal of Haematology. 1992 ; Vol. 81, No. 2. pp. 203-211.
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AU - De Totero, D.

AU - Falini, B.

AU - Lemoli, R. M.

AU - Soria, M. R.

AU - Pileri, S.

AU - Gobbi, M.

AU - Stein, H.

AU - Flenghi, L.

AU - Martelli, M. F.

AU - Stirpe, F.

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