Beta defensin-2 is reduced in central but not in distal airways of smoker COPD patients

Elisabetta Pace, Maria Ferraro, Marta Ida Minervini, Patrizio Vitulo, Loredana Pipitone, Giuseppina Chiappara, Liboria Siena, Angela Marina Montalbano, Malcolm Johnson, Mark Gjomarkaj

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Abstract

Background: Altered pulmonary defenses in chronic obstructive pulmonary disease (COPD) may promote distal airways bacterial colonization. The expression/activation of Toll Like receptors (TLR) and beta 2 defensin (HBD2) release by epithelial cells crucially affect pulmonary defence mechanisms. Methods: The epithelial expression of TLR4 and of HBD2 was assessed in surgical specimens from current smokers COPD (s-COPD; n = 17), ex-smokers COPD (ex-s-COPD; n = 8), smokers without COPD (S; n = 12), and from non-smoker non-COPD subjects (C; n = 13). Results: In distal airways, s-COPD highly expressed TLR4 and HBD2. In central airways, S and s-COPD showed increased TLR4 expression. Lower HBD2 expression was observed in central airways of s-COPD when compared to S and to ex-s-COPD. s-COPD had a reduced HBD2 gene expression as demonstrated by real-time PCR on micro-dissected bronchial epithelial cells. Furthermore, HBD2 expression positively correlated with FEV1/FVC ratio and inversely correlated with the cigarette smoke exposure. In a bronchial epithelial cell line (16 HBE) IL-1β significantly induced the HBD2 mRNA expression and cigarette smoke extracts significantly counteracted this IL-1 mediated effect reducing both the activation of NFkB pathway and the interaction between NFkB and HBD2 promoter. Conclusions: This study provides new insights on the possible mechanisms involved in the alteration of innate immunity mechanisms in COPD.

Original languageEnglish
Article numbere33601
JournalPLoS One
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 16 2012

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beta-Defensins
Pulmonary diseases
respiratory tract diseases
Chronic Obstructive Pulmonary Disease
Epithelial Cells
epithelial cells
cigarettes
interleukin-1
Interleukin-1
smoke
Smoke
Tobacco Products
Chemical activation
lungs
Obstructive Lung Diseases
Toll-Like Receptor 2
Lung
Defensins
bacterial colonization
Toll-Like Receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pace, E., Ferraro, M., Minervini, M. I., Vitulo, P., Pipitone, L., Chiappara, G., ... Gjomarkaj, M. (2012). Beta defensin-2 is reduced in central but not in distal airways of smoker COPD patients. PLoS One, 7(3), [e33601]. https://doi.org/10.1371/journal.pone.0033601

Beta defensin-2 is reduced in central but not in distal airways of smoker COPD patients. / Pace, Elisabetta; Ferraro, Maria; Minervini, Marta Ida; Vitulo, Patrizio; Pipitone, Loredana; Chiappara, Giuseppina; Siena, Liboria; Montalbano, Angela Marina; Johnson, Malcolm; Gjomarkaj, Mark.

In: PLoS One, Vol. 7, No. 3, e33601, 16.03.2012.

Research output: Contribution to journalArticle

Pace, E, Ferraro, M, Minervini, MI, Vitulo, P, Pipitone, L, Chiappara, G, Siena, L, Montalbano, AM, Johnson, M & Gjomarkaj, M 2012, 'Beta defensin-2 is reduced in central but not in distal airways of smoker COPD patients', PLoS One, vol. 7, no. 3, e33601. https://doi.org/10.1371/journal.pone.0033601
Pace, Elisabetta ; Ferraro, Maria ; Minervini, Marta Ida ; Vitulo, Patrizio ; Pipitone, Loredana ; Chiappara, Giuseppina ; Siena, Liboria ; Montalbano, Angela Marina ; Johnson, Malcolm ; Gjomarkaj, Mark. / Beta defensin-2 is reduced in central but not in distal airways of smoker COPD patients. In: PLoS One. 2012 ; Vol. 7, No. 3.
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AU - Pipitone, Loredana

AU - Chiappara, Giuseppina

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