The availability of cryopreserved hepatocytes is required for a more widespread use of hepatocyte transplantation, but human hepatocytes are easily damaged during freezing-thawing. Here, preincubation with unconjugated bilirubin, a physiological antioxidant, resulted in increased viability and function of hepatocytes (as determined by trypan blue exclusion, mitochondrial succinate dehydrogenases activity, urea synthesis, and cytochrome P450 1A/2) compared with cells incubated without the pigment. These findings suggest that unconjugated bilirubin may be used as cryoprotectant in clinical hepatocyte transplantation.
|Number of pages||3|
|Journal||Journal of Pediatric Gastroenterology and Nutrition|
|Publication status||Published - Jun 2010|
- Hepatocyte transplantation
- Liver metabolism
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health