Bilirubin inhibits the TNFα-related induction of three endothelial adhesion molecules

Graciela Luján Mazzone, Igino Rigato, J. Donald Ostrow, Fleur Bossi, Alessia Bortoluzzi, Caecilia H C Sukowati, Francesco Tedesco, Claudio Tiribelli

Research output: Contribution to journalArticle

Abstract

Since an increased serum unconjugated bilirubin (UCB) level has been proposed as an independent protective factor against atherosclerotic disease, we investigated the molecular events at the basis of this effect. HUVEC and H5V cells were treated with TNFα and UCB and the effects assessed on E-selectin, VCAM-1 and ICAM-1. In HUVEC cells, UCB blunted the TNFα-induced gene upregulation of E-selectin VCAM-1 and ICAM-1. The same pattern was observed in H5V cells except for ICAM-1. UCB also inhibited the PMN endothelial adhesion in HUVEC H5V cells. Western blot and FACS analysis confirmed that UCB prevented TNFα-induced over-expression of adhesion molecules proteins in H5V cells. These data contribute to further explain the protective effect of bilirubin against development of atherosclerosis.

Original languageEnglish
Pages (from-to)338-344
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number2
DOIs
Publication statusPublished - Aug 21 2009

Keywords

  • Adhesion molecules
  • Atherosclerosis
  • Bilirubin
  • Endothelial cell activation
  • TNFα

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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  • Cite this

    Mazzone, G. L., Rigato, I., Ostrow, J. D., Bossi, F., Bortoluzzi, A., Sukowati, C. H. C., Tedesco, F., & Tiribelli, C. (2009). Bilirubin inhibits the TNFα-related induction of three endothelial adhesion molecules. Biochemical and Biophysical Research Communications, 386(2), 338-344. https://doi.org/10.1016/j.bbrc.2009.06.029