Bimodal spectroscopy of formalin fixed samples to discriminate dysplastic and tumor brain tissues

S. Anand, R. Cicchi, F. Giordano, A. M. Buccoliero, R. Guerrini, F. S. Pavone

Research output: Contribution to journalArticle

Abstract

Biomedical spectroscopy has gained attention in the past few years for disease diagnosis. Fluorescence and Raman spectroscopies provide finger-print information related to biochemical and morphological alterations when tissues progress from the normal to a malignant stage. Usually, freshly excised tissue specimens are preferred for bio-spectroscopic studies. However, ethical issues, sample availability and distance between the surgery room and the laboratory provide an impelling restriction for in-vitro spectroscopic studies using freshly excised samples. After surgical resection tissues are fixed in 4% formalin for histological studies under a light microscope. The process of fixation prevents degradation of tissues. In this study, we probe the use of formalin fixed sample for differentiating normal and dysplastic brain tissues using fluorescence and Raman spectroscopies. It was found that fluorescence spectral profile changes in the wavelength range from 550-750 nm between dysplastic and tumor samples. Also, significant differences were found in the Raman spectral profiles of such samples. The results indicate a potential diagnostic application of spectroscopy in formalin fixed brain samples for differentiating dysplastic and tumor brain tissues.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalLatvian Journal of Physics and Technical Sciences
Volume51
Issue number5
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Brain tumor
  • Dysplasia
  • Fluorescence
  • Formalin fixation
  • Raman spectroscopy

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Engineering(all)

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