Abstract
Modulation of the interaction between cellular or viral transcription factors and target DNA sequences may represent a potential experimental strategy to control proliferation of neoplastic cells as cell as virus DNA replication. Distamycin represents a likely candidate to mediate such modulation by pharmacological means. In order to obtain more detailed information on structure-activity relationships of these compounds, we have analysed the effects of distamycin and two distamycin analogues on the binding of a recombinant protein, the Epstein-Barr virus nuclear antigen 1 (EBNA-1) to its target sequence of Epstein-Barr virus, containing the 12 bp palindromic consensus TAGCATATGCTA. The sequence selectivity in the binding of distamycin to DNA was evaluated by footprinting experiments, while the effects of distamycins on DNA-protein interactions was analysed by means of electrophoretic mobility shift assay. The data presented in this paper suggest that distamycin and its analogues differentially inhibit the interaction between DNA-binding proteins and target DNA sequences.
| Original language | English |
|---|---|
| Pages (from-to) | 143-149 |
| Number of pages | 7 |
| Journal | European Journal of Pharmacology: Molecular Pharmacology |
| Volume | 267 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 15 1994 |
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Keywords
- DNA-binding drug
- Epstein-Barr virus
- Transcription factors
ASJC Scopus subject areas
- Pharmacology
Cite this
Binding of Epstein-Barr virus nuclear antigen 1 to DNA : inhibition by distamycin and two novel distamycin analogues. / Feriotto, Giordana; Ciucci, Alessandra; Mischiati, Carlo; Animati, Fabio; Lombardi, Paolo; Giacomini, Patrizio; Arcamone, Federico; Gambari, Roberto.
In: European Journal of Pharmacology: Molecular Pharmacology, Vol. 267, No. 2, 15.04.1994, p. 143-149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Binding of Epstein-Barr virus nuclear antigen 1 to DNA
T2 - inhibition by distamycin and two novel distamycin analogues
AU - Feriotto, Giordana
AU - Ciucci, Alessandra
AU - Mischiati, Carlo
AU - Animati, Fabio
AU - Lombardi, Paolo
AU - Giacomini, Patrizio
AU - Arcamone, Federico
AU - Gambari, Roberto
PY - 1994/4/15
Y1 - 1994/4/15
N2 - Modulation of the interaction between cellular or viral transcription factors and target DNA sequences may represent a potential experimental strategy to control proliferation of neoplastic cells as cell as virus DNA replication. Distamycin represents a likely candidate to mediate such modulation by pharmacological means. In order to obtain more detailed information on structure-activity relationships of these compounds, we have analysed the effects of distamycin and two distamycin analogues on the binding of a recombinant protein, the Epstein-Barr virus nuclear antigen 1 (EBNA-1) to its target sequence of Epstein-Barr virus, containing the 12 bp palindromic consensus TAGCATATGCTA. The sequence selectivity in the binding of distamycin to DNA was evaluated by footprinting experiments, while the effects of distamycins on DNA-protein interactions was analysed by means of electrophoretic mobility shift assay. The data presented in this paper suggest that distamycin and its analogues differentially inhibit the interaction between DNA-binding proteins and target DNA sequences.
AB - Modulation of the interaction between cellular or viral transcription factors and target DNA sequences may represent a potential experimental strategy to control proliferation of neoplastic cells as cell as virus DNA replication. Distamycin represents a likely candidate to mediate such modulation by pharmacological means. In order to obtain more detailed information on structure-activity relationships of these compounds, we have analysed the effects of distamycin and two distamycin analogues on the binding of a recombinant protein, the Epstein-Barr virus nuclear antigen 1 (EBNA-1) to its target sequence of Epstein-Barr virus, containing the 12 bp palindromic consensus TAGCATATGCTA. The sequence selectivity in the binding of distamycin to DNA was evaluated by footprinting experiments, while the effects of distamycins on DNA-protein interactions was analysed by means of electrophoretic mobility shift assay. The data presented in this paper suggest that distamycin and its analogues differentially inhibit the interaction between DNA-binding proteins and target DNA sequences.
KW - DNA-binding drug
KW - Epstein-Barr virus
KW - Transcription factors
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U2 - 10.1016/0922-4106(94)90165-1
DO - 10.1016/0922-4106(94)90165-1
M3 - Article
VL - 267
SP - 143
EP - 149
JO - European Journal of Pharmacology - Molecular Pharmacology Section
JF - European Journal of Pharmacology - Molecular Pharmacology Section
SN - 0922-4106
IS - 2
ER -