Binding of glucose to the D-galactose/D-glucose-binding protein from Escherichia coli restores the native protein secondary structure and thermostability that are lost upon calcium depletion

Sabato D'Auria, Alessio Ausili, Anna Marabotti, Antonio Varriale, Viviana Scognamiglio, Maria Staiano, Enrico Bertoli, Mosè Rossi, Fabio Tanfani

Research output: Contribution to journalArticle

Abstract

The effect of the depletion of calcium on the structure and thermal stability of the D-galactose/D-glucose-binding protein (GGBP) from Escherichia coli was studied by fluorescence spectroscopy and Fourier-transform infrared spectroscopy. The calcium-depleted protein (GGBP-Ca) was also studied in the presence of glucose (GGBP-Ca/Glc). The results show that calcium depletion has a small effect on the secondary structure of GGBP, and, in particular it affects a population of α-helices with a low exposure to solvent. Alternatively, glucose-binding to GGBP-Ca eliminates the effect induced by calcium depletion by restoring a secondary structure similar to that of the native protein. In addition, the infrared and fluorescence data obtained reveal that calcium depletion markedly reduces the thermal stability of GGBP. In particular, the spectroscopic experiments show that the depletion of calcium mainly affects the stability of the C-terminal domain of the protein. However, the binding of glucose restores the thermal stability of GGBP-Ca. The thermostability of GGBP and GGBP-Ca was also studied by molecular dynamics simulations. The simulation data support the spectroscopic results. New insights into the role of calcium in the thermal stability of GGBP contribute to a better understanding of the protein function and constitute important information for the development of biotechnological applications of this protein. Mutations and/or labelling of amino acid residues located in the protein C-terminal domain may affect the stability of the whole protein structure.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalJournal of Biochemistry
Volume139
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Secondary Protein Structure
Galactose
Escherichia coli
Carrier Proteins
Calcium
Glucose
Proteins
Thermodynamic stability
Hot Temperature
Protein Stability
Fluorescence Spectrometry
Fluorescence spectroscopy
Fourier Transform Infrared Spectroscopy
Molecular Dynamics Simulation
Protein C
Labeling
Molecular dynamics

Keywords

  • Galactose/glucose-binding protein
  • Infrared spectroscopy
  • Protein stability
  • Protein structure

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of glucose to the D-galactose/D-glucose-binding protein from Escherichia coli restores the native protein secondary structure and thermostability that are lost upon calcium depletion. / D'Auria, Sabato; Ausili, Alessio; Marabotti, Anna; Varriale, Antonio; Scognamiglio, Viviana; Staiano, Maria; Bertoli, Enrico; Rossi, Mosè; Tanfani, Fabio.

In: Journal of Biochemistry, Vol. 139, No. 2, 02.2006, p. 213-221.

Research output: Contribution to journalArticle

D'Auria, Sabato ; Ausili, Alessio ; Marabotti, Anna ; Varriale, Antonio ; Scognamiglio, Viviana ; Staiano, Maria ; Bertoli, Enrico ; Rossi, Mosè ; Tanfani, Fabio. / Binding of glucose to the D-galactose/D-glucose-binding protein from Escherichia coli restores the native protein secondary structure and thermostability that are lost upon calcium depletion. In: Journal of Biochemistry. 2006 ; Vol. 139, No. 2. pp. 213-221.
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