Binding of IL-1β to IL-1R type II at single cell level

Antonella Stoppacciaro, Paola Bossu', Paolo Ghiara, Luigi P. Ruco, Stefano Censini, Giuseppe Scapigliati, Sandra Nuti, Aldo Tagliabue, Carlo D. Baroni, Diana Boraschi

Research output: Contribution to journalArticle

Abstract

To gain information on the possible biologic role of IL-1R type II (IL-1RII), expression of the 68-kDa IL-1 binding protein on human lymphoblastoid B cells was investigated at single cell level. Binding of iodinated IL-1β was evaluated by autoradiography on cytosmears of IL-1RII positive B cell lines RAJI, the RAJI clone 1H7, and STS 25. Results obtained suggest an heterogeneity of IL-1RII expression within the B cell population, with only 5 to 16% of the cells able to bind IL-1β. Up-regulation of IL-1RII expression by dexamethasone, evident in conventional binding assays, was achieved through both increase in the number of IL-1 binding cells (14-30%) and augmentation of receptor density on positive cells. By combining autoradiography with immunocytochemical staining, it could be shown that about 80% of IL-1RII+ cells were negative for Ki67, a nuclear antigen expressed from late G1 to M phase. Cell cycle dependent expression of IL-1RII was confirmed on cells enriched in different phases of the cell cycle by counterflow centrifugal elutriation. It is thus proposed that IL-1RII is associated to the cell cycle.

Original languageEnglish
Pages (from-to)1561-1566
Number of pages6
JournalJournal of Immunology
Volume147
Issue number5
Publication statusPublished - Sep 1 1991

ASJC Scopus subject areas

  • Immunology

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    Stoppacciaro, A., Bossu', P., Ghiara, P., Ruco, L. P., Censini, S., Scapigliati, G., Nuti, S., Tagliabue, A., Baroni, C. D., & Boraschi, D. (1991). Binding of IL-1β to IL-1R type II at single cell level. Journal of Immunology, 147(5), 1561-1566.