Binding of low-molecular-weight heparin to aortic endothelium in rabbits

G. F. Gensini, A. Fortini, A. Lombardi, E. Pesciullesi, C. Pieroni, G. G. Neri Serneri

Research output: Contribution to journalArticle

Abstract

The ability of a low-molecular-weight heparin to bind in vivo to the aortic endothelium in rabbits, compared with that of unfractionated extractive sodium heparin (UHEP), was investigated. For this purpose a low-molecular-weight heparin (LMWHEP) fraction (CY 216 A, 4,000-5,000 daltons) and an extractive UHEP (average 17,000 daltons) were labeled with 99mTc and injected (0.2, 0.6, and 1.2 mg/kg) intravenously in groups of 6 rabbits. The binding to endothelium was then measured by counting the radioactivity of samples of abdominal aorta. Both heparins bound to endothelium with a fast, saturable, and reversible binding. No significant difference in the total bound radioactivity was found between the two heparins, suggesting that the investigated LMWHEP retains the ability to bind to the endothelium, contributing to its athrombogenic properties.

Original languageEnglish
Pages (from-to)466-472
Number of pages7
JournalPathophysiology of Haemostasis and Thrombosis
Volume14
Issue number6
DOIs
Publication statusPublished - 1984

Keywords

  • Anti-Xa activity
  • Binding sites
  • Endothelium
  • Low-molecular-weight heparin

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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  • Cite this

    Gensini, G. F., Fortini, A., Lombardi, A., Pesciullesi, E., Pieroni, C., & Neri Serneri, G. G. (1984). Binding of low-molecular-weight heparin to aortic endothelium in rabbits. Pathophysiology of Haemostasis and Thrombosis, 14(6), 466-472. https://doi.org/10.1159/000215107