Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene: Effects of IFN-γ on tumor cell lines

R. Gambari, R. Barbieri, C. Nastruzzi, G. Feriotto, R. Piva, P. G. Natali, P. Giacomini

Research output: Contribution to journalArticle

Abstract

The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalJournal of Biological Regulators and Homeostatic Agents
Volume4
Issue number3
Publication statusPublished - 1990

Fingerprint

consensus sequence
Consensus Sequence
HLA-DR Antigens
interferons
Tumor Cell Line
Interferons
cell lines
Genes
genes
nuclear proteins
Nuclear Proteins
binding proteins
Carrier Proteins
HLA-D Antigens
Artificial Cells
Cell Line
oligonucleotides
neoplasm cells
Oligonucleotides
cultured cells

ASJC Scopus subject areas

  • Immunology
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Physiology (medical)
  • Medicine (miscellaneous)
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene : Effects of IFN-γ on tumor cell lines. / Gambari, R.; Barbieri, R.; Nastruzzi, C.; Feriotto, G.; Piva, R.; Natali, P. G.; Giacomini, P.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 4, No. 3, 1990, p. 98-106.

Research output: Contribution to journalArticle

@article{ef146803fde14bac89733a627df66dd1,
title = "Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene: Effects of IFN-γ on tumor cell lines",
abstract = "The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.",
author = "R. Gambari and R. Barbieri and C. Nastruzzi and G. Feriotto and R. Piva and Natali, {P. G.} and P. Giacomini",
year = "1990",
language = "English",
volume = "4",
pages = "98--106",
journal = "Journal of Biological Regulators and Homeostatic Agents",
issn = "0393-974X",
publisher = "Biolife s.a.s.",
number = "3",

}

TY - JOUR

T1 - Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene

T2 - Effects of IFN-γ on tumor cell lines

AU - Gambari, R.

AU - Barbieri, R.

AU - Nastruzzi, C.

AU - Feriotto, G.

AU - Piva, R.

AU - Natali, P. G.

AU - Giacomini, P.

PY - 1990

Y1 - 1990

N2 - The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.

AB - The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.

UR - http://www.scopus.com/inward/record.url?scp=0025648584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025648584&partnerID=8YFLogxK

M3 - Article

C2 - 2135380

AN - SCOPUS:0025648584

VL - 4

SP - 98

EP - 106

JO - Journal of Biological Regulators and Homeostatic Agents

JF - Journal of Biological Regulators and Homeostatic Agents

SN - 0393-974X

IS - 3

ER -