Abstract
The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.
| Original language | English |
|---|---|
| Pages (from-to) | 98-106 |
| Number of pages | 9 |
| Journal | Journal of Biological Regulators and Homeostatic Agents |
| Volume | 4 |
| Issue number | 3 |
| Publication status | Published - 1990 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Physiology (medical)
- Medicine (miscellaneous)
- Physiology
- Agricultural and Biological Sciences(all)
Cite this
Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene : Effects of IFN-γ on tumor cell lines. / Gambari, R.; Barbieri, R.; Nastruzzi, C.; Feriotto, G.; Piva, R.; Natali, P. G.; Giacomini, P.
In: Journal of Biological Regulators and Homeostatic Agents, Vol. 4, No. 3, 1990, p. 98-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Binding of nuclear factors of the IFN-γ consensus sequence of the human HLA-DRα gene
T2 - Effects of IFN-γ on tumor cell lines
AU - Gambari, R.
AU - Barbieri, R.
AU - Nastruzzi, C.
AU - Feriotto, G.
AU - Piva, R.
AU - Natali, P. G.
AU - Giacomini, P.
PY - 1990
Y1 - 1990
N2 - The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.
AB - The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon(IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-BI/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-BI/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-BI/B2 is not modulated by IFN-γ treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DRα gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.
UR - http://www.scopus.com/inward/record.url?scp=0025648584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025648584&partnerID=8YFLogxK
M3 - Article
C2 - 2135380
AN - SCOPUS:0025648584
VL - 4
SP - 98
EP - 106
JO - Journal of Biological Regulators and Homeostatic Agents
JF - Journal of Biological Regulators and Homeostatic Agents
SN - 0393-974X
IS - 3
ER -