Binding of Streptococcus gordonii to extracellular matrix proteins

Barbara Giomarelli; Livia Visai; Karolin Hijazi; Simonetta Rindi; Michela Ponzio; Francesco Iannelli; Pietro Speziale; Gianni Pozzi

doi:10.1111/j.1574-6968.2006.00479.x

Binding of Streptococcus gordonii to extracellular matrix proteins

Barbara Giomarelli, Livia Visai, Karolin Hijazi, Simonetta Rindi, Michela Ponzio, Francesco Iannelli, Pietro Speziale, Gianni Pozzi

Istituti Clinici Scientifici Maugeri Spa – Società Benefit

Research output: Contribution to journal › Article › peer-review

Abstract

Knock-out mutants of Streptococcus gordonii Challis were constructed and assayed for binding to extracellular matrix proteins (EMPs) by enzyme-linked immunosorbent assay (ELISA). It was shown that (i) the mutant lacking the cell wall polysaccharide receptor could no longer bind type I and type II collagen, (ii) the mutant lacking the fibronectin-binding proteins CshA and FbpA was also strongly impaired in collagen binding and (iii) the mutant lacking the methionine sulfoxide reductase MsrA was significantly impaired in fibronectin binding. Our results indicate that binding to EMPs by S. gordonii is a multifactorial process controlled by genes located at three different chromosomal sites.

Original language	English
Pages (from-to)	172-177
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	265
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2006.00479.x
Publication status	Published - Dec 2006

Keywords

Extracellular matrix proteins (EMPs)
Microbial adhesins
Microbial surface components recognizing adhesive matrix molecules (MSCRAMMs)
Oral streptococci
Streptococcus gordonii

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

Access to Document

10.1111/j.1574-6968.2006.00479.x

Fingerprint Dive into the research topics of 'Binding of Streptococcus gordonii to extracellular matrix proteins'. Together they form a unique fingerprint.

Cite this

@article{3b5d9941821c4078a1e7f76170220381,

title = "Binding of Streptococcus gordonii to extracellular matrix proteins",

abstract = "Knock-out mutants of Streptococcus gordonii Challis were constructed and assayed for binding to extracellular matrix proteins (EMPs) by enzyme-linked immunosorbent assay (ELISA). It was shown that (i) the mutant lacking the cell wall polysaccharide receptor could no longer bind type I and type II collagen, (ii) the mutant lacking the fibronectin-binding proteins CshA and FbpA was also strongly impaired in collagen binding and (iii) the mutant lacking the methionine sulfoxide reductase MsrA was significantly impaired in fibronectin binding. Our results indicate that binding to EMPs by S. gordonii is a multifactorial process controlled by genes located at three different chromosomal sites.",

keywords = "Extracellular matrix proteins (EMPs), Microbial adhesins, Microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), Oral streptococci, Streptococcus gordonii",

author = "Barbara Giomarelli and Livia Visai and Karolin Hijazi and Simonetta Rindi and Michela Ponzio and Francesco Iannelli and Pietro Speziale and Gianni Pozzi",

year = "2006",

month = dec,

doi = "10.1111/j.1574-6968.2006.00479.x",

language = "English",

volume = "265",

pages = "172--177",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Binding of Streptococcus gordonii to extracellular matrix proteins

AU - Giomarelli, Barbara

AU - Visai, Livia

AU - Hijazi, Karolin

AU - Rindi, Simonetta

AU - Ponzio, Michela

AU - Iannelli, Francesco

AU - Speziale, Pietro

AU - Pozzi, Gianni

PY - 2006/12

Y1 - 2006/12

N2 - Knock-out mutants of Streptococcus gordonii Challis were constructed and assayed for binding to extracellular matrix proteins (EMPs) by enzyme-linked immunosorbent assay (ELISA). It was shown that (i) the mutant lacking the cell wall polysaccharide receptor could no longer bind type I and type II collagen, (ii) the mutant lacking the fibronectin-binding proteins CshA and FbpA was also strongly impaired in collagen binding and (iii) the mutant lacking the methionine sulfoxide reductase MsrA was significantly impaired in fibronectin binding. Our results indicate that binding to EMPs by S. gordonii is a multifactorial process controlled by genes located at three different chromosomal sites.

AB - Knock-out mutants of Streptococcus gordonii Challis were constructed and assayed for binding to extracellular matrix proteins (EMPs) by enzyme-linked immunosorbent assay (ELISA). It was shown that (i) the mutant lacking the cell wall polysaccharide receptor could no longer bind type I and type II collagen, (ii) the mutant lacking the fibronectin-binding proteins CshA and FbpA was also strongly impaired in collagen binding and (iii) the mutant lacking the methionine sulfoxide reductase MsrA was significantly impaired in fibronectin binding. Our results indicate that binding to EMPs by S. gordonii is a multifactorial process controlled by genes located at three different chromosomal sites.

KW - Extracellular matrix proteins (EMPs)

KW - Microbial adhesins

KW - Microbial surface components recognizing adhesive matrix molecules (MSCRAMMs)

KW - Oral streptococci

KW - Streptococcus gordonii

UR - http://www.scopus.com/inward/record.url?scp=33751212442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751212442&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2006.00479.x

DO - 10.1111/j.1574-6968.2006.00479.x

M3 - Article

C2 - 17038048

AN - SCOPUS:33751212442

VL - 265

SP - 172

EP - 177

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -

Binding of Streptococcus gordonii to extracellular matrix proteins

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this