TY - JOUR
T1 - Bioactivity and bone healing properties of biomimetic porous composite scaffold
T2 - In vitro and in vivo studies
AU - Veronesi, Francesca
AU - Giavaresi, Gianluca
AU - Guarino, Vincenzo
AU - Raucci, Maria Grazia
AU - Sandri, Monica
AU - Tampieri, Anna
AU - Ambrosio, Luigi
AU - Fini, Milena
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Tissue engineering (TE) represents a valid alternative to traditional surgical therapies for the management of bone defects that do not regenerate spontaneously. Scaffolds, one of the most important component of TE strategy, should be biocompatible, bioactive, osteoconductive, and osteoinductive. The aim of this study was to evaluate the biological properties and bone regeneration ability of a porous poly(ε-caprolactone) (PCL) scaffold, incorporating MgCO3-doped hydroxyapatite particles, uncoated (PCL-MgCHA) or coated by apatite-like crystals via biomimetic treatment (PCL-MgCHAB). It was observed that both scaffolds are not cytotoxic and, even if cell viability was similar on both scaffolds, PCL-MgCHAB showed higher alkaline phosphatase and collagen I (COLL I) production at day 7. PCL-MgCHA induced more tumor necrosis factor-α release than PCL-MgCHAB, while osteocalcin was produced less by both scaffolds up to 7 days and no significant differences were observed for transforming growth factor-β synthesis. The percentage of new bone trabeculae growth in wide defects carried out in rabbit femoral distal epiphyses was significantly higher in PCL-MgCHAB in comparison with PCL-MgCHA at 4 weeks and even more at 12 weeks after implantation. This study highlighted the role of a biomimetic composite scaffold in bone regeneration and lays the foundations for its future employment in the clinical practice.
AB - Tissue engineering (TE) represents a valid alternative to traditional surgical therapies for the management of bone defects that do not regenerate spontaneously. Scaffolds, one of the most important component of TE strategy, should be biocompatible, bioactive, osteoconductive, and osteoinductive. The aim of this study was to evaluate the biological properties and bone regeneration ability of a porous poly(ε-caprolactone) (PCL) scaffold, incorporating MgCO3-doped hydroxyapatite particles, uncoated (PCL-MgCHA) or coated by apatite-like crystals via biomimetic treatment (PCL-MgCHAB). It was observed that both scaffolds are not cytotoxic and, even if cell viability was similar on both scaffolds, PCL-MgCHAB showed higher alkaline phosphatase and collagen I (COLL I) production at day 7. PCL-MgCHA induced more tumor necrosis factor-α release than PCL-MgCHAB, while osteocalcin was produced less by both scaffolds up to 7 days and no significant differences were observed for transforming growth factor-β synthesis. The percentage of new bone trabeculae growth in wide defects carried out in rabbit femoral distal epiphyses was significantly higher in PCL-MgCHAB in comparison with PCL-MgCHA at 4 weeks and even more at 12 weeks after implantation. This study highlighted the role of a biomimetic composite scaffold in bone regeneration and lays the foundations for its future employment in the clinical practice.
KW - biomaterials
KW - bone cells
KW - bone regeneration
KW - composite scaffolds
KW - histomorphometry
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U2 - 10.1002/jbm.a.35433
DO - 10.1002/jbm.a.35433
M3 - Article
C2 - 25689266
AN - SCOPUS:84938215781
VL - 103
SP - 2932
EP - 2941
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 9
ER -