Bioassay of growth-stimulating activity of serum: Comparison of lymphocyte and cartilage assays in normal and growth hormone-deficient children

Mauro Bozzola, Roue Marie Schimpff, Jacques Charrier, Francesco Severi, Jean Claude Job

Research output: Contribution to journalArticle

Abstract

The somatomedin and/or growth-stimulating activity of serum from hypopitui-tary children and short children with normal growth hormone (GH) response to stimulation tests were studied using different bioassays; thymidine incorporation into human activated lymphocytes; sulfate incorporation into chick embryo cartilage: and simultaneous thymidine uptake into the same cartilages. The results showed that lymphocyte assay is highly sensitive to small amounts of serum and is GH-dependenl in children with low GH secretion. On the contrary, the cartilage assays need higher serum concentration and their GH-dcpendence appears only in subjects with normal or low-normal GH secretion. The lack of correlation between the results of the three bioassays suggests that they measure both somatomedins and different scrum factors involved in the regulation of growth.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
JournalHormone Research in Paediatrics
Volume21
Issue number2
DOIs
Publication statusPublished - 1985

Keywords

  • Growth
  • Growth hormone deficiency
  • Somatomedin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Pediatrics, Perinatology, and Child Health

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