Biochemical activity and multiple locations of particulate guanylate cyclase in Rhyacophila dorsalis acutidens (Insecta: Trichoptera) provide insights into the cGMP signalling pathway in Malpighian tubules

T. Secca, M. Sciaccaluga, A. Marra, L. Barberini, M. C. Bicchierai

Research output: Contribution to journalArticle

Abstract

In insect renal physiology, cGMP and cAMP have important regulatory roles. In Drosophila melanogaster, considered a good model for molecular physiology studies, and in other insects, cGMP and cAMP act as signalling molecules in the Malpighian tubules (MTs).However, many questions related to cyclic nucleotide functions are unsolved in principal cells (PC) and stellate cells (SC), the two cell types that compose the MT. In PC, despite the large body of information available on soluble guanylate cyclase (sGC) in the cGMP pathway, the functional circuit of particulate guanylate cyclase (pGC) remains obscure. In SC, on the other side, the synthesis and physiological role of the cGMP are still unknown. Our biochemical data regarding the presence of cyclic nucleotides in the MTs of Rhyacophila dorsalis acutidens revealed a cGMP level above the 50%, in comparison with the cAMP. The specific activity values for the membrane-bound guanylate cyclase were also recorded, implying that, besides the sGC, pGC is a physiologically relevant source of cGMP in MTs. Cytochemical studies showed ultrastructurally that there was a great deal of pGC on the basolateral membranes of both the principal and stellate cells. In addition, pGC was also detected in the contact zone between the two cell types and in the apical microvillar region of the stellate cells bordering the tubule lumen. The pGC signal is so well represented in PC and, unexpectedly in SC of MTs, that it is possible to hypothesize the existence of still uncharacterized physiological processes regulated by the pGC-cGMP system.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalJournal of Insect Physiology
Volume57
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Rhyacophila
Malpighian Tubules
guanylate cyclase
Malpighian tubules
Guanylate Cyclase
Trichoptera
Insecta
Insects
particulates
cells
cyclic nucleotides
Cyclic Nucleotides
physiology
Physiological Phenomena
molecular models
insects
Molecular Models
Membranes
Drosophila melanogaster

Keywords

  • Biochemical assay
  • CGMP
  • Cytochemical assay
  • Malpighian tubules
  • Particulate guanylate cyclase
  • Principal cells
  • Rhyacophila
  • Stellate cells

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Biochemical activity and multiple locations of particulate guanylate cyclase in Rhyacophila dorsalis acutidens (Insecta : Trichoptera) provide insights into the cGMP signalling pathway in Malpighian tubules. / Secca, T.; Sciaccaluga, M.; Marra, A.; Barberini, L.; Bicchierai, M. C.

In: Journal of Insect Physiology, Vol. 57, No. 4, 04.2011, p. 521-528.

Research output: Contribution to journalArticle

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abstract = "In insect renal physiology, cGMP and cAMP have important regulatory roles. In Drosophila melanogaster, considered a good model for molecular physiology studies, and in other insects, cGMP and cAMP act as signalling molecules in the Malpighian tubules (MTs).However, many questions related to cyclic nucleotide functions are unsolved in principal cells (PC) and stellate cells (SC), the two cell types that compose the MT. In PC, despite the large body of information available on soluble guanylate cyclase (sGC) in the cGMP pathway, the functional circuit of particulate guanylate cyclase (pGC) remains obscure. In SC, on the other side, the synthesis and physiological role of the cGMP are still unknown. Our biochemical data regarding the presence of cyclic nucleotides in the MTs of Rhyacophila dorsalis acutidens revealed a cGMP level above the 50{\%}, in comparison with the cAMP. The specific activity values for the membrane-bound guanylate cyclase were also recorded, implying that, besides the sGC, pGC is a physiologically relevant source of cGMP in MTs. Cytochemical studies showed ultrastructurally that there was a great deal of pGC on the basolateral membranes of both the principal and stellate cells. In addition, pGC was also detected in the contact zone between the two cell types and in the apical microvillar region of the stellate cells bordering the tubule lumen. The pGC signal is so well represented in PC and, unexpectedly in SC of MTs, that it is possible to hypothesize the existence of still uncharacterized physiological processes regulated by the pGC-cGMP system.",
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