Biochemical analysis of HLA class I subunits expression in breast cancer tissues

Maria Pia Pistillo, Guido Nicolò, Sandra Salvi, Paolo Capanni, Luisa Perdelli, Gennaro Pasciucco, Giovanni Battista Ferrara

Research output: Contribution to journalArticle

Abstract

The expression of HLA class I α-chain and β2-m subunits was studied at the protein level by a semiquantitative Western blot (WB) approach, in 25 primary breast tumors. The results indicated three pathways of alterations defined comparing the tumor WB gel band with the corresponding PBL gel band: (i) high downregulation pattern (the tumor WB gel band was ≤50% relative to the PBL band), which was found in 44% and 36% of tumors for α-chain and β2-m, respectively; (ii) low downregulation pattern (the tumor gel band was between 51% and 75%), which was found in 24% and 20% of tumors for α-chain and β2-m, respectively; and (iii) absent downregulation pattern (the tumor gel band was >75%), which was found in 32% and 44% of tumors for α-chain and β2-m, respectively. The concordance rate with immunohistochemistry (IHC) performed on the same tissue samples was 72% for α-chain and 64% for β2-m. This study shows that the use of a semiquantitative WB technique can well define the levels of HLA class I antigens in an autologous setting allowing the biochemical analysis of HLA class I downregulation directly in solid tumor tissues. In addition, the WB technique can be a valuable tool to objectively support the IHC method. (C) American Society for Histocompatibility and Immunogenerics, 2000.

Original languageEnglish
Pages (from-to)397-407
Number of pages11
JournalHuman Immunology
Volume61
Issue number4
DOIs
Publication statusPublished - Apr 2000

Keywords

  • Densitometry
  • HLA-class I subunits
  • Immunohistochemistry
  • Monoclonal antibodies
  • Western blot

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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  • Cite this

    Pistillo, M. P., Nicolò, G., Salvi, S., Capanni, P., Perdelli, L., Pasciucco, G., & Ferrara, G. B. (2000). Biochemical analysis of HLA class I subunits expression in breast cancer tissues. Human Immunology, 61(4), 397-407. https://doi.org/10.1016/S0198-8859(99)00179-2