Biochemical and antigenic characterization of CD45 polypeptides expressed on plasma membrane and internal granules of human neutrophils

Rafael Pulido, Pedro Lacal, Faustino Mollinedo, Francisco Sánchez-Madrid

Research output: Contribution to journalArticle

Abstract

The expression of CD45 polypeptides, a phosphotyrosine phosphatase complex specific of leukocytes, has been investigated in both resting and activated neutrophils by using anti-CD45 monoclonal antibodies (MAb) which specifically recognize different polypeptide components of the CD45 molecular complex. Polypeptides of 180 and 130-150 kDa were equally precipitated by either a conventional CD45 MAb recognizing an antigenic determinant shared by the four CD45 glycoproteins (220, 205, 190 and 180 kDa) or by the anti-180 kDa UCHL1 MAb. These polypeptides were overexpressed on neutrophil plasma membranes after degranulatory stimulation. Conversely, neither the anti-220 kDa CD45R nor anti-220/205/190 kDa MAb reacted with CD45 molecules from resting or activated neutrophils. Furthermore, permeabilization analysis and comparative immunoprecipitation studies with different anti-CD45 MAb from fractions enriched in various neutrophil granules revealed that CD45 polypeptides (180 and 130-150 kDa) from internal granules are antigenic and biochemically identical to those expressed on plasma membrane.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalFEBS Letters
Volume249
Issue number2
DOIs
Publication statusPublished - Jun 5 1989

Fingerprint

Cell membranes
Neutrophils
Monoclonal Antibodies
Cell Membrane
Peptides
Protein Tyrosine Phosphatases
Immunoprecipitation
Epitopes
Glycoproteins
Leukocytes
Molecules

Keywords

  • (Human neutrophil)
  • Antigen specificity
  • Differentiation antigen
  • Phosphotyrosine phosphatase
  • Polypeptide, CD45

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Biochemical and antigenic characterization of CD45 polypeptides expressed on plasma membrane and internal granules of human neutrophils. / Pulido, Rafael; Lacal, Pedro; Mollinedo, Faustino; Sánchez-Madrid, Francisco.

In: FEBS Letters, Vol. 249, No. 2, 05.06.1989, p. 337-342.

Research output: Contribution to journalArticle

Pulido, Rafael ; Lacal, Pedro ; Mollinedo, Faustino ; Sánchez-Madrid, Francisco. / Biochemical and antigenic characterization of CD45 polypeptides expressed on plasma membrane and internal granules of human neutrophils. In: FEBS Letters. 1989 ; Vol. 249, No. 2. pp. 337-342.
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