Biochemical and functional characterization of the interaction between pentraxin 3 and C1q

Alma J. Nauta, Barbara Bottazzi, Alberto Mantovani, Giovanni Salvatori, Uday Kishore, Wilhelm J. Schwaeble, Alexandre R. Gingras, Sotiria Tzima, Fernando Vivanco, Jesús Egido, Odette Tijsma, Erik C. Hack, Mohamed R. Daha, Anja Roos

Research output: Contribution to journalArticle

Abstract

Pentraxin 3 (PTX3) is a recently characterized member of the pentraxin family of acute-phase proteins produced during inflammation. Classical short pentraxins, C-reactive protein, and serum amyloid P component can bind to C1q and thereby activate the classical complement pathway. Since PTX3 can also bind C1q, the present study was designed to define the interaction between PTX3 and C1q and to examine the functional consequences of this interaction. A dose-dependent binding of both C1q and the C1 complex to PTX3 was observed. Experiments with recombinant globular head domains of human C1q A, B, and C chains indicated that C1q interacts with PTX3 via its globular head region. Binding of C1q to immobilized PTX3 induced activation of the classical complement pathway as assessed by C4 deposition. Furthermore, PTX3 enhanced C1q binding and complement activation on apoptotic cells. However, in the fluid-phase, pre-incubation of PTX3 with C1q resulted in inhibition of complement activation by blocking the interaction of C1q with immunoglobulins. These results indicate that PTX3 can both inhibit and activate the classical complement pathway by binding C1q, depending on the way it is presented. PTX3 may therefore be involved in the regulation of the innate immune response.

Original languageEnglish
Pages (from-to)465-473
Number of pages9
JournalEuropean Journal of Immunology
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Classical Complement Pathway
Complement Activation
Serum Amyloid P-Component
PTX3 protein
Acute-Phase Proteins
Innate Immunity
C-Reactive Protein
Immunoglobulins
Inflammation

Keywords

  • C1q
  • Complement activation
  • PTX3

ASJC Scopus subject areas

  • Immunology

Cite this

Biochemical and functional characterization of the interaction between pentraxin 3 and C1q. / Nauta, Alma J.; Bottazzi, Barbara; Mantovani, Alberto; Salvatori, Giovanni; Kishore, Uday; Schwaeble, Wilhelm J.; Gingras, Alexandre R.; Tzima, Sotiria; Vivanco, Fernando; Egido, Jesús; Tijsma, Odette; Hack, Erik C.; Daha, Mohamed R.; Roos, Anja.

In: European Journal of Immunology, Vol. 33, No. 2, 01.02.2003, p. 465-473.

Research output: Contribution to journalArticle

Nauta, AJ, Bottazzi, B, Mantovani, A, Salvatori, G, Kishore, U, Schwaeble, WJ, Gingras, AR, Tzima, S, Vivanco, F, Egido, J, Tijsma, O, Hack, EC, Daha, MR & Roos, A 2003, 'Biochemical and functional characterization of the interaction between pentraxin 3 and C1q', European Journal of Immunology, vol. 33, no. 2, pp. 465-473. https://doi.org/10.1002/immu.200310022
Nauta, Alma J. ; Bottazzi, Barbara ; Mantovani, Alberto ; Salvatori, Giovanni ; Kishore, Uday ; Schwaeble, Wilhelm J. ; Gingras, Alexandre R. ; Tzima, Sotiria ; Vivanco, Fernando ; Egido, Jesús ; Tijsma, Odette ; Hack, Erik C. ; Daha, Mohamed R. ; Roos, Anja. / Biochemical and functional characterization of the interaction between pentraxin 3 and C1q. In: European Journal of Immunology. 2003 ; Vol. 33, No. 2. pp. 465-473.
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