Biochemical and molecular investigations on qualitative and quantitative Hb polymorphism in the river buffalo (Bubalus bubalis L.) population reared in Southern Italy

Mario Iorio, Donatella Vincenti, Mario Annunziata, Rosario Rullo, Raffaele Bonamassa, Aldo Di Luccia, Elisa Pieragostini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

On 398 river buffalo samples, randomly collected in distinct breeding areas of the Campania region, high-resolution analytical systems were used to identify both qualitative and quantitative variations of the Hb phenotype. Polyacrylamide gel isoelectric focusing and HPLC were used to determine the ratio between HBA1 and HBA2 globin chains; restriction endonuclease analysis was performed to assess whether quantitative variations in Hb bands were related to an unusual number of α-globin genes. In the two buffalo subpopulations, allele frequencies of the alpha and beta globin systems were calculated, and F statistics (FIS, FIT and FST) were estimated as parameters of genetic diversity. The results suggest that: i) as shown by RFLP analysis, only a couple of associated α globin genes account for the quantitative variations recorded at the phenotypic level; ii) as expected, in the α globin gene system (HBA), the frequency of haplotype B (HBA-B) largely exceeded that of haplotype A (HBA-A) (95.1% vs 4.9%); iii) the frequency of the usual allele at the beta locus is 0.6, as opposed to 0.4 of the slow variant; iiii) the most significant component of variation of the genetic system of hemoglobin is between individuals within the same location. Copyright by the Brazilian Society of Genetics.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalGenetics and Molecular Biology
Volume27
Issue number2
Publication statusPublished - 2004

Fingerprint

Globins
Buffaloes
Rivers
Italy
Gene Frequency
Haplotypes
Population
Genes
alpha-Globins
beta-Globins
DNA Restriction Enzymes
Isoelectric Focusing
Restriction Fragment Length Polymorphisms
Breeding
Hemoglobins
High Pressure Liquid Chromatography
Phenotype

Keywords

  • Gene frequencies
  • Globin genes
  • Haplotype
  • Phenotype
  • RFLP

ASJC Scopus subject areas

  • Genetics

Cite this

Biochemical and molecular investigations on qualitative and quantitative Hb polymorphism in the river buffalo (Bubalus bubalis L.) population reared in Southern Italy. / Iorio, Mario; Vincenti, Donatella; Annunziata, Mario; Rullo, Rosario; Bonamassa, Raffaele; Di Luccia, Aldo; Pieragostini, Elisa.

In: Genetics and Molecular Biology, Vol. 27, No. 2, 2004, p. 167-173.

Research output: Contribution to journalArticle

Iorio, Mario ; Vincenti, Donatella ; Annunziata, Mario ; Rullo, Rosario ; Bonamassa, Raffaele ; Di Luccia, Aldo ; Pieragostini, Elisa. / Biochemical and molecular investigations on qualitative and quantitative Hb polymorphism in the river buffalo (Bubalus bubalis L.) population reared in Southern Italy. In: Genetics and Molecular Biology. 2004 ; Vol. 27, No. 2. pp. 167-173.
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