Biochemical and morphological changes in isolated rabbit hearts after prolonged hypothermic ischaemia: comparison of two cardioplegic solutions.

R. Poltronieri, A. Cevese, R. Bregu, A. Motta, C. Zancanaro, P. Bernocchi

Research output: Contribution to journalArticle

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Abstract

This work evaluates the myocardial protective potential of potassium cardioplegia on ischaemically arrested and reperfused hearts by two cardioplegic solutions: the University of Wisconsin solution (UW) and the standard crystalloid solution of St. Thomas' Hospital (ST). Evaluation of myocardial preservation was based on creatine kinase and lactate releases and on high-energy phosphate preservation of isolated rabbit hearts after 4 hours' hypothermic ischaemia. A morphometric ultrastructural evaluation of mitochondria in cardiomyocytes was also performed. The hearts of 24 rabbits were normothermally perfused with oxygenated Krebs-Henseleit solution for 30 min (Langendroff preparation), and the baseline contractile performance and biochemical parameters were evaluated. The hearts were then arrested and stored in the cardioplegic solutions (12 UW and 12 ST) at 4 degrees C for 4 hours. The hearts were then rewarmed and reperfused with oxygenated Krebs-Henseleit solution for further 30 min. At the end of reperfusion, creatine phosphate and high energy phosphates were higher with UW (p <0.05); creatine kinase release during reperfusion was significantly lower with UW both at 15 min (p <0.01) and at 30 min (p <0.05). Lactate release during the first 15 min of reperfusion was about doubled (p <0.05) with respect to controls in both groups; at 30 min this increase had almost vanished (+8%) with UW but not with ST (+30%). Ultrastructural morphometry did not show any significant difference at the level of mitochondria between the two treatments. The results indicate, for UW, an improved myocardial preservation associated with relative retention of high-energy phosphates and higher recovery of mechanical function, accelerated metabolic recovery and reduced stress of cell membranes.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalPhysiological Research
Volume44
Issue number3
Publication statusPublished - 1995

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Cardioplegic Solutions
Ischemia
Rabbits
Reperfusion
Phosphates
Creatine Kinase
Lactic Acid
Mitochondria
Induced Heart Arrest
Phosphocreatine
Recovery of Function
Cardiac Myocytes
Potassium
Cell Membrane

ASJC Scopus subject areas

  • Physiology

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Biochemical and morphological changes in isolated rabbit hearts after prolonged hypothermic ischaemia : comparison of two cardioplegic solutions. / Poltronieri, R.; Cevese, A.; Bregu, R.; Motta, A.; Zancanaro, C.; Bernocchi, P.

In: Physiological Research, Vol. 44, No. 3, 1995, p. 157-164.

Research output: Contribution to journalArticle

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