Biochemical characterization of TEM-92 extended-spectrum β-lactamase, a protein differing from TEM-52 in the signal peptide

Mariagrazia Perilli, Bernardetta Segatore, Maria Rosaria De Massis, Laura Pagani, Francesco Luzzaro, Gian Maria Rossolini, Gianfranco Amicosante

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A blaTEM-92 gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.

Original languageEnglish
Pages (from-to)3981-3983
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

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Cephamycins
Moxalactam
Cephalosporins
Protein Sorting Signals
Aztreonam
Sulbactam
Proteus mirabilis
Clavulanic Acid
Enzymes
Penicillins
Proteins
Hydrolysis
Escherichia coli
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Biochemical characterization of TEM-92 extended-spectrum β-lactamase, a protein differing from TEM-52 in the signal peptide. / Perilli, Mariagrazia; Segatore, Bernardetta; De Massis, Maria Rosaria; Pagani, Laura; Luzzaro, Francesco; Rossolini, Gian Maria; Amicosante, Gianfranco.

In: Antimicrobial Agents and Chemotherapy, Vol. 46, No. 12, 01.12.2002, p. 3981-3983.

Research output: Contribution to journalArticle

Perilli, Mariagrazia ; Segatore, Bernardetta ; De Massis, Maria Rosaria ; Pagani, Laura ; Luzzaro, Francesco ; Rossolini, Gian Maria ; Amicosante, Gianfranco. / Biochemical characterization of TEM-92 extended-spectrum β-lactamase, a protein differing from TEM-52 in the signal peptide. In: Antimicrobial Agents and Chemotherapy. 2002 ; Vol. 46, No. 12. pp. 3981-3983.
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