Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis

Luisa Borgianni, Filomena De Luca, Maria Cristina Thaller, Yunsop Chong, Gian Maria Rossolini, Jean Denis Docquier

Research output: Contribution to journalArticlepeer-review

Abstract

The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.

Original languageEnglish
Pages (from-to)1755-1758
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

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