Biochemical detection of novel anaplastic lymphoma kinase proteins in tissue sections of anaplastic large cell lymphoma

Karen Pulford, Brunangelo Falini, Jaqueline Cordell, Andreas Rosenwald, German Ott, Hans Konrad Müller-Hermelink, Kenneth A. MacLennan, Laurence Lamant, Antonio Carbone, Elias Campo, David Y. Mason

Research output: Contribution to journalArticle

Abstract

The (2;5) translocation, found in many T-cell and null cell anaplastic large cell lymphomas (ALCLs), creates a hybrid gene encoding the 80-kd NPM- ALK protein. Typically neoplastic cells show labeling of both nucleus and cytoplasm for anaplastic lymphoma kinase (ALK) and for the N-terminus of nucleophosmin (NPM). However, 10-20% of cases exhibit cytoplasmic labeling only for ALK, indicating the probable presence of variants of the classical (2;5) translocation that do not involve the NPM gene. We report the detection (using Western blotting and an in vitro kinase assay) in seven such ALCL cases, of ALK proteins with molecular masses of 85 kd, 97 kd (one case exhibiting a (2;3)(p23;q21) translocation), 104 kd (one case carried a (1;2)(q21;p23) translocation), and 113 kd. Tyrosine kinase activity was detected in four of these proteins, but the N-terminal portion of NPM could not be detected. These results show how ALCL cases that express ALK proteins other than NPM-ALK can be detected by sensitive biochemical techniques using routine cryostat sections.

Original languageEnglish
Pages (from-to)1657-1663
Number of pages7
JournalAmerican Journal of Pathology
Volume154
Issue number6
Publication statusPublished - Jun 1999

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Pulford, K., Falini, B., Cordell, J., Rosenwald, A., Ott, G., Müller-Hermelink, H. K., MacLennan, K. A., Lamant, L., Carbone, A., Campo, E., & Mason, D. Y. (1999). Biochemical detection of novel anaplastic lymphoma kinase proteins in tissue sections of anaplastic large cell lymphoma. American Journal of Pathology, 154(6), 1657-1663.