Biochemical evidence of a physical interaction between Sulfolobus solfataricus B-family and Y-family DNA polymerases

Mariarita De Felice, Barbara Medagli, Luca Esposito, Mariarosaria De Falco, Biagio Pucci, Mosè Rossi, Petr Grùz, Takehiko Nohmi, Francesca M. Pisani

Research output: Contribution to journalArticlepeer-review

Abstract

The hyper-thermophilic archaeon Sulfolobus solfataricus possesses two functional DNA polymerases belonging to the B-family (Sso DNA pol B1) and to the Y-family (Sso DNA pol Y1). Sso DNA pol B1 recognizes the presence of uracil and hypoxanthine in the template strand and stalls synthesis 3-4 bases upstream of this lesion ("read-ahead" function). On the other hand, Sso DNA pol Y1 is able to synthesize across these and other lesions on the template strand. Herein we report evidence that Sso DNA pol B1 physically interacts with DNA pol Y1 by surface plasmon resonance measurements and immuno-precipitation experiments. The region of DNA pol B1 responsible for this interaction has been mapped in the central portion of the polypeptide chain (from the amino acid residue 482 to 617), which includes an extended protease hyper-sensitive linker between the N- and C-terminal modules (amino acid residues Asn482-Ala497) and the α-helices forming the "fingers" sub-domain (α-helices R, R′ and S). These results have important implications for understanding the polymerase-switching mechanism on the damaged template strand during genome replication in S. solfataricus.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalExtremophiles
Volume11
Issue number2
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Archaea
  • DNA polymerase
  • DNA replication
  • Genome stability
  • Sulfolobus solfataricus
  • Translesion synthesis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Fingerprint Dive into the research topics of 'Biochemical evidence of a physical interaction between Sulfolobus solfataricus B-family and Y-family DNA polymerases'. Together they form a unique fingerprint.

Cite this