Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with leber's hereditary optic neuropathy

V. Carelli, A. Ghelli, L. Bucchi, P. Montagna, A. De Negri, V. Leuzzi, C. Carducci, G. Lenaz, E. Lugaresi, M. Degli Esposti

Research output: Contribution to journalArticle

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Abstract

We report the effect on complex I function of the 14484 Leber's hereditary optic neuropathy (LHON) mutation affecting the ND6 subunit gene. The same gene was also reported to carry another mutation, at position 14459, associated with the LHON/dystonia phenotype that induces a reduction of complex I-specific activity and increases the sensitivity to the product decylubiquinol. Given the proximity of both mutations in the ND6 gene, we tested the specific activity of complex I and its sensitivity to myxothiazol and nonylbenzoquinol, both inhibitors at the ubiquinol product site, in platelet submitochondrial particles from nine 14484 homoplasmic individuals, 8 Italians with Caucasian mtDNA haplogroup J (adjunctive 4216 and 13708 mutations), and 1 Tunisian with an African mtDNA haplogroup. The specific activity of complex I was not affected by the 14484 mutation, but the sensitivity to both inhibitors was significantly increased compared with control subjects regardless of the presence of haplogroup J polymorphisms. Analysis of 70 different amino acid sequences of the ND6 subunit indicated that the 14484 mutation affects an amino acid belonging to its most conserved region, which shows local similarities with cytochrome b regions interacting with ubiquinone or ubiquinol in complex III. Our results suggest that both 14484 and 14459 mutations may affect amino acids forming the interaction site of ubiquinol product, and the 14485 mutation produces a biochemical defect resembling in part that already reported for the common 11778/ND4 LHON mutation.

Original languageEnglish
Pages (from-to)320-328
Number of pages9
JournalAnnals of Neurology
Volume45
Issue number3
DOIs
Publication statusPublished - 1999

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Leber's Hereditary Optic Atrophy
Mitochondrial DNA
Point Mutation
Mutation
Submitochondrial Particles
Genes
Amino Acids
Cytochromes b
Ubiquinone
Electron Transport Complex III
Amino Acid Sequence
Blood Platelets
Phenotype

ASJC Scopus subject areas

  • Neuroscience(all)

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Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with leber's hereditary optic neuropathy. / Carelli, V.; Ghelli, A.; Bucchi, L.; Montagna, P.; De Negri, A.; Leuzzi, V.; Carducci, C.; Lenaz, G.; Lugaresi, E.; Degli Esposti, M.

In: Annals of Neurology, Vol. 45, No. 3, 1999, p. 320-328.

Research output: Contribution to journalArticle

Carelli, V, Ghelli, A, Bucchi, L, Montagna, P, De Negri, A, Leuzzi, V, Carducci, C, Lenaz, G, Lugaresi, E & Degli Esposti, M 1999, 'Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with leber's hereditary optic neuropathy', Annals of Neurology, vol. 45, no. 3, pp. 320-328. https://doi.org/10.1002/1531-8249(199903)45:3<320::AID-ANA7>3.0.CO;2-L
Carelli, V. ; Ghelli, A. ; Bucchi, L. ; Montagna, P. ; De Negri, A. ; Leuzzi, V. ; Carducci, C. ; Lenaz, G. ; Lugaresi, E. ; Degli Esposti, M. / Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with leber's hereditary optic neuropathy. In: Annals of Neurology. 1999 ; Vol. 45, No. 3. pp. 320-328.
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AU - Ghelli, A.

AU - Bucchi, L.

AU - Montagna, P.

AU - De Negri, A.

AU - Leuzzi, V.

AU - Carducci, C.

AU - Lenaz, G.

AU - Lugaresi, E.

AU - Degli Esposti, M.

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