TY - JOUR
T1 - Biocompatibility assessment of haemodialysis membrane materials by proteomic investigations
AU - Pieroni, Luisa
AU - Levi Mortera, Stefano
AU - Greco, Viviana
AU - Sirolli, Vittorio
AU - Ronci, Maurizio
AU - Felaco, Paolo
AU - Fucci, Giorgio
AU - De Fulviis, Silvia
AU - Massoud, Renato
AU - Condò, Stefano
AU - Capria, Ambrogio
AU - Di Daniele, Nicola
AU - Bernardini, Sergio
AU - Urbani, Andrea
AU - Bonomini, Mario
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The exposure of blood to an artificial surface such as the haemodialysis membrane results in the nearly instantaneous deposition of a layer of plasma proteins. The composition of the protein layer profoundly influences all subsequent events, and to a large extent determines the biocompatibility of the biomaterial. In the present study, we examine the protein adsorption capacity and coagulation profiles of the polysulfone-based helixone material in comparison to cellulose triacetate. A differential profiling investigation using shotgun proteomics data-independent analysis was applied to eluates obtained with each membrane after a dialysis session, in order to assess the function of desorbed proteins. Functional classification and network analysis performed using bioinformatics tools shed light on the involvement of adsorbed proteins into important molecular processes, such as lipid transport and metabolism, cell growth differentiation and communication, and the coagulation cascade. The collected evidence was further validated by targeted mass spectrometry using selected reaction monitoring on proteotypic transitions of key protein effectors, confirming the different panels of adsorbed protein on each membrane. The coagulation profile during haemodialysis of patients under polysulfone-based helixone filter cartridges was also assessed showing a slightly higher platelet activation profile after the dialysis session. The overall collected evidence highlights a modulation of the coagulation biological pathway during haemodialysis, which is largely influenced by the biomaterial used.
AB - The exposure of blood to an artificial surface such as the haemodialysis membrane results in the nearly instantaneous deposition of a layer of plasma proteins. The composition of the protein layer profoundly influences all subsequent events, and to a large extent determines the biocompatibility of the biomaterial. In the present study, we examine the protein adsorption capacity and coagulation profiles of the polysulfone-based helixone material in comparison to cellulose triacetate. A differential profiling investigation using shotgun proteomics data-independent analysis was applied to eluates obtained with each membrane after a dialysis session, in order to assess the function of desorbed proteins. Functional classification and network analysis performed using bioinformatics tools shed light on the involvement of adsorbed proteins into important molecular processes, such as lipid transport and metabolism, cell growth differentiation and communication, and the coagulation cascade. The collected evidence was further validated by targeted mass spectrometry using selected reaction monitoring on proteotypic transitions of key protein effectors, confirming the different panels of adsorbed protein on each membrane. The coagulation profile during haemodialysis of patients under polysulfone-based helixone filter cartridges was also assessed showing a slightly higher platelet activation profile after the dialysis session. The overall collected evidence highlights a modulation of the coagulation biological pathway during haemodialysis, which is largely influenced by the biomaterial used.
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U2 - 10.1039/c5mb00058k
DO - 10.1039/c5mb00058k
M3 - Article
C2 - 25845767
AN - SCOPUS:84934875380
VL - 11
SP - 1633
EP - 1643
JO - Molecular BioSystems
JF - Molecular BioSystems
SN - 1742-206X
IS - 6
ER -