Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture

Daniela Moretti, Mattia Lorenzo DiFrancesco, Parikshit Pratim Sharma, Silvia Dante, Edoardo Albisetti, Marco Monticelli, Riccardo Bertacco, Daniela Petti, Pietro Baldelli, Fabio Benfenati

Research output: Contribution to journalArticle

Abstract

Magnetoencephalography has been established nowadays as a crucial in vivo technique for clinical and diagnostic applications due to its unprecedented spatial and temporal resolution and its non-invasive methods. However, the innate nature of the biomagnetic signals derived from active biological tissue is still largely unknown. One alternative possibility for in vitro analysis is the use of magnetic sensor arrays based on Magnetoresistance. However, these sensors have never been used to perform long-term in vitro studies mainly due to critical biocompatibility issues with neurons in culture. In this study, we present the first biomagnetic chip based on magnetic tunnel junction (MTJ) technology for cell culture studies and show the biocompatibility of these sensors. We obtained a full biocompatibility of the system through the planarization of the sensors and the use of a three-layer capping of SiO2/Si3N4/SiO2. We grew primary neurons up to 20 days on the top of our devices and obtained proper functionality and viability of the overlying neuronal networks. At the same time, MTJ sensors kept their performances unchanged for several weeks in contact with neurons and neuronal medium. These results pave the way to the development of high performing biomagnetic sensing technology for the electrophysiology of in vitro systems, in analogy with Multi Electrode Arrays.

Original languageEnglish
Pages (from-to)909
JournalFrontiers in Neuroscience
Volume12
DOIs
Publication statusPublished - 2018

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Neurons
Technology
Magnetoencephalography
Electrophysiology
Electrodes
Cell Culture Techniques
Equipment and Supplies
In Vitro Techniques
silicon nitride

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Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture. / Moretti, Daniela; DiFrancesco, Mattia Lorenzo; Sharma, Parikshit Pratim; Dante, Silvia; Albisetti, Edoardo; Monticelli, Marco; Bertacco, Riccardo; Petti, Daniela; Baldelli, Pietro; Benfenati, Fabio.

In: Frontiers in Neuroscience, Vol. 12, 2018, p. 909.

Research output: Contribution to journalArticle

Moretti, D, DiFrancesco, ML, Sharma, PP, Dante, S, Albisetti, E, Monticelli, M, Bertacco, R, Petti, D, Baldelli, P & Benfenati, F 2018, 'Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture', Frontiers in Neuroscience, vol. 12, pp. 909. https://doi.org/10.3389/fnins.2018.00909
Moretti, Daniela ; DiFrancesco, Mattia Lorenzo ; Sharma, Parikshit Pratim ; Dante, Silvia ; Albisetti, Edoardo ; Monticelli, Marco ; Bertacco, Riccardo ; Petti, Daniela ; Baldelli, Pietro ; Benfenati, Fabio. / Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture. In: Frontiers in Neuroscience. 2018 ; Vol. 12. pp. 909.
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