TY - JOUR
T1 - Biocompatibility of cluster-assembled nanostructured TiO2 with primary and cancer cells
AU - Carbone, Roberta
AU - Marangi, Ida
AU - Zanardi, Andrea
AU - Giorgetti, Luca
AU - Chierici, Elisabetta
AU - Berlanda, Giuseppe
AU - Podestà, Alessandro
AU - Fiorentini, Francesca
AU - Bongiorno, Gero
AU - Piseri, Paolo
AU - Pelicci, Pier Giuseppe
AU - Milani, Paolo
PY - 2006/6
Y1 - 2006/6
N2 - We have characterized the biocompatibility of nanostructured TiO 2 films produced by the deposition of a supersonic beam of TiO x clusters. Physical analysis shows that these films possess, at the nanoscale, a granularity and porosity mimicking those of typical extracellular matrix structures and adsorption properties that could allow surface functionalization with different macromolecules such as DNA, proteins, and peptides. To explore the biocompatibility of this novel nanostructured surface, different cancer and primary cells were analyzed in terms of morphological appearance (by bright field microscopy and immunofluorescence) and growth properties, with the aim to evaluate cluster-assembled TiO2 films as substrates for cell-based and tissue-based applications. Our results strongly suggest that this new biomaterial supports normal growth and adhesion of primary and cancer cells with no need for coating with ECM proteins; we thus propose this new material as an optimal substrate for different applications in cell-based assays, biosensors or microfabricated medical devices.
AB - We have characterized the biocompatibility of nanostructured TiO 2 films produced by the deposition of a supersonic beam of TiO x clusters. Physical analysis shows that these films possess, at the nanoscale, a granularity and porosity mimicking those of typical extracellular matrix structures and adsorption properties that could allow surface functionalization with different macromolecules such as DNA, proteins, and peptides. To explore the biocompatibility of this novel nanostructured surface, different cancer and primary cells were analyzed in terms of morphological appearance (by bright field microscopy and immunofluorescence) and growth properties, with the aim to evaluate cluster-assembled TiO2 films as substrates for cell-based and tissue-based applications. Our results strongly suggest that this new biomaterial supports normal growth and adhesion of primary and cancer cells with no need for coating with ECM proteins; we thus propose this new material as an optimal substrate for different applications in cell-based assays, biosensors or microfabricated medical devices.
KW - Biocompatibility
KW - Biomimetic material
KW - Cell adhesion
KW - Extracellular matrix
KW - Nanotopography
KW - Titanium
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U2 - 10.1016/j.biomaterials.2006.01.056
DO - 10.1016/j.biomaterials.2006.01.056
M3 - Article
C2 - 16504283
AN - SCOPUS:33644791752
VL - 27
SP - 3221
EP - 3229
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 17
ER -