Biocompatibility of poly(d,l-lactide-co-glycolide) nanoparticles conjugated with alendronate

Elisabetta Cenni, Donatella Granchi, Sofia Avnet, Caterina Fotia, Manuela Salerno, Dorotea Micieli, Maria G. Sarpietro, Rosario Pignatello, Francesco Castelli, Nicola Baldini

Research output: Contribution to journalArticlepeer-review

Abstract

Nanoparticles made of a conjugate of poly(d,l-lactide-co-glycolide) with alendronate (PLGA-ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30-35%. PLGA-ALE NPs size, evaluated by photon correlation spectroscopy, was 198.7 ± 0.2 nm. Haemocompatibility studies using different concentrations of PLGA-ALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 μg/ml of PLGA-ALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGA-ALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGA-ALE NP- and PBS-added media. These results demonstrated that PLGA-ALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration.

Original languageEnglish
Pages (from-to)1400-1411
Number of pages12
JournalBiomaterials
Volume29
Issue number10
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Bisphosphonate
  • Blood compatibility
  • Drug delivery
  • Nanoparticle
  • Poly(lactic-co-glycolic acid) copolymer

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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