Abstract
We extended for the first time pulsed laser ablation to the deposition of octacalcium phosphate Ca8H2(PO4) 6·5H2O (OCP) thin films. The depositions were performed with a pulsed UV laser source (λ=248nm, τ≥20ns) in a flux of hot water vapors. The targets were sintered from crystalline OCP powder and the laser ablation fluence was set at values of 1.5-2J/cm2. During depositions the collectors, Si or Ti substrates, were maintained at a constant temperature within the range 20-200°C. The resulting structures were submitted to heat treatment in hot water vapors for up to 6h. The best results were obtained at a substrate temperature of 150°C during both deposition and post-deposition treatment. High-resolution electron microscopy and XRD at grazing incidence indicated that the coatings obtained were made of nanocrystalline OCP. Cross-section TEM investigations showed that the coatings contained droplets stacked on Ti substrates as well as distributed across the entire thickness of the arborescence-like structure layers. The results of WST-1 assay, cell adherence, DNA replication, and caspase-1 activity confirmed the good biocompatibility of the coatings.
Original language | English |
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Pages (from-to) | 2539-2545 |
Number of pages | 7 |
Journal | Biomaterials |
Volume | 25 |
Issue number | 13 |
DOIs | |
Publication status | Published - Jun 2004 |
Keywords
- Calcium phosphate coating
- In vitro test
- Laser ablation
- Octacalcium phosphate
- TEM
- XRD
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering