Biocompatible nanocrystalline octacalcium phosphate thin films obtained by pulsed laser deposition

G. Socol, P. Torricelli, B. Bracci, M. Iliescu, F. Miroiu, A. Bigi, J. Werckmann, I. N. Mihailescu

Research output: Contribution to journalArticlepeer-review


We extended for the first time pulsed laser ablation to the deposition of octacalcium phosphate Ca8H2(PO4) 6·5H2O (OCP) thin films. The depositions were performed with a pulsed UV laser source (λ=248nm, τ≥20ns) in a flux of hot water vapors. The targets were sintered from crystalline OCP powder and the laser ablation fluence was set at values of 1.5-2J/cm2. During depositions the collectors, Si or Ti substrates, were maintained at a constant temperature within the range 20-200°C. The resulting structures were submitted to heat treatment in hot water vapors for up to 6h. The best results were obtained at a substrate temperature of 150°C during both deposition and post-deposition treatment. High-resolution electron microscopy and XRD at grazing incidence indicated that the coatings obtained were made of nanocrystalline OCP. Cross-section TEM investigations showed that the coatings contained droplets stacked on Ti substrates as well as distributed across the entire thickness of the arborescence-like structure layers. The results of WST-1 assay, cell adherence, DNA replication, and caspase-1 activity confirmed the good biocompatibility of the coatings.

Original languageEnglish
Pages (from-to)2539-2545
Number of pages7
Issue number13
Publication statusPublished - Jun 2004


  • Calcium phosphate coating
  • In vitro test
  • Laser ablation
  • Octacalcium phosphate
  • TEM
  • XRD

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


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