Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring.

A. B. Lovati, E. Vianello, G. Talò, C. Recordati, L. Bonizzi, E. Galliera, M. Broggini, M. Moretti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.

Original languageEnglish
JournalJournal of Biological Regulators and Homeostatic Agents
Volume25
Issue number2 Suppl
Publication statusPublished - Apr 2011

Fingerprint

Nanoparticles
Magnetic Resonance Spectroscopy
Transplantation
Cell Tracking
Chondrocytes
ferric oxide

ASJC Scopus subject areas

  • Oncology
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)
  • Immunology and Allergy
  • Immunology
  • Endocrinology
  • Physiology
  • Cancer Research

Cite this

Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring. / Lovati, A. B.; Vianello, E.; Talò, G.; Recordati, C.; Bonizzi, L.; Galliera, E.; Broggini, M.; Moretti, M.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 25, No. 2 Suppl, 04.2011.

Research output: Contribution to journalArticle

@article{f4aecb544c034d0f9d5955eaefa422f7,
title = "Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring.",
abstract = "Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.",
author = "Lovati, {A. B.} and E. Vianello and G. Tal{\`o} and C. Recordati and L. Bonizzi and E. Galliera and M. Broggini and M. Moretti",
year = "2011",
month = "4",
language = "English",
volume = "25",
journal = "Journal of Biological Regulators and Homeostatic Agents",
issn = "0393-974X",
publisher = "Biolife s.a.s.",
number = "2 Suppl",

}

TY - JOUR

T1 - Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring.

AU - Lovati, A. B.

AU - Vianello, E.

AU - Talò, G.

AU - Recordati, C.

AU - Bonizzi, L.

AU - Galliera, E.

AU - Broggini, M.

AU - Moretti, M.

PY - 2011/4

Y1 - 2011/4

N2 - Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.

AB - Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.

UR - http://www.scopus.com/inward/record.url?scp=84855181529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855181529&partnerID=8YFLogxK

M3 - Article

C2 - 22051172

AN - SCOPUS:84855181529

VL - 25

JO - Journal of Biological Regulators and Homeostatic Agents

JF - Journal of Biological Regulators and Homeostatic Agents

SN - 0393-974X

IS - 2 Suppl

ER -