Biodistribution studies of 99mTc-labeled myoblasts in a murine model of muscular dystrophy

F. R. Colombo, Y. Torrente, R. Casati, R. Benti, S. Corti, S. Salani, M. G. D'Angelo, A. DeLiso, G. Scarlato, N. Bresolin, P. Gerundini

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was twofold: first, to evaluate the myoblast labeling of various 99mTc complexes and to select the complex that best accomplishes this labeling, and second to evaluate the biodistribution of myoblasts labeled with this complex using mice with MDX muscular dystrophy (the murine homologue of Duchenne's muscular dystrophy). The following ligands were used to prepare the corresponding 99mTc complexes: hexakis-methoxy-isobutyl-isonitrile (MIBI), bis(2-ethoxyethyl)diphosphinoethane (Tf), (RR,SS)-4,8-diaza-3,6,6,9-tetramethyl-undecane-2,10-dione-bisoxime (HM-PAO), bis(N-ethyl)dithiocarbamate (NEt), and bis(N-ethoxy, N-ethyl)dithiocarbamate (NOEt). One million murine myoblasts were incubated for 30-60 minutes with 5 mCi of each of the 99mTc complexes prepared from the above ligands. Viability was assessed by microscopic counting after trypan blue staining, and the radioactivity absorbed in the cells was measured after centrifugation. The compound with the highest uptake in cellular pellets was [99mTc]N-NOEt. The biodistribution of myoblasts labeled with this complex was evaluated after intraaortic injection in dystrophic mice. Such an approach has the potential of effecting widespread gene transfer through the bloodstream to muscles lacking dystrophin.

Original languageEnglish
Pages (from-to)935-940
Number of pages6
JournalNuclear Medicine and Biology
Volume28
Issue number8
DOIs
Publication statusPublished - 2001

Keywords

  • 99mTc
  • Dystrophy
  • Gene therapy
  • Myoblast transplantation

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

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