Bioenergetics in aging: Mitochondrial proton leak in aging rat liver, kidney and heart

G. Serviddio, F. Bellanti, A. D. Romano, R. Tamborra, T. Rollo, E. Altomare, G. Vendemiale

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aging is associated with a decline in performance in many organs and loss of physiological performance can be due to free radicals. Mitochondria are incompletely coupled: during oxidative phosphorylation some of the redox energy is dissipated as natural proton leak across the inner membrane. To verify whether proton leak occurs in mitochondria during aging, we measured the mitochondrial respiratory chain activity, membrane potential and proton leak in liver, kidneys and heart of young and old rats. Mitochondria from old rats showed normal rates of Complex I and Complex II respiration. However, they had a lower membrane potential compared to mitochondria from younger rats. In addition, they exhibited an increased rate of proton conductance which partially dissipated the mitochondrial membrane potential when the rate of electron transport was suppressed. This could compromise energy homeostasis in aging cells in conditions that require additional energy supply and could minimize oxidative damage to DNA.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalRedox Report
Volume12
Issue number1-2
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Mitochondria
Liver
Energy Metabolism
Protons
Rats
Aging of materials
Membranes
Kidney
Electron Transport
Membrane Potentials
Mitochondrial Membrane Potential
Cell Aging
Oxidative Phosphorylation
DNA Damage
Oxidation-Reduction
Free Radicals
Respiration
Homeostasis
DNA

Keywords

  • Aging
  • Membrane potential
  • Mitochondria
  • Proton conductance
  • Respiratory chain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Serviddio, G., Bellanti, F., Romano, A. D., Tamborra, R., Rollo, T., Altomare, E., & Vendemiale, G. (2007). Bioenergetics in aging: Mitochondrial proton leak in aging rat liver, kidney and heart. Redox Report, 12(1-2), 91-95. https://doi.org/10.1179/135100007X162112

Bioenergetics in aging : Mitochondrial proton leak in aging rat liver, kidney and heart. / Serviddio, G.; Bellanti, F.; Romano, A. D.; Tamborra, R.; Rollo, T.; Altomare, E.; Vendemiale, G.

In: Redox Report, Vol. 12, No. 1-2, 02.2007, p. 91-95.

Research output: Contribution to journalArticle

Serviddio, G, Bellanti, F, Romano, AD, Tamborra, R, Rollo, T, Altomare, E & Vendemiale, G 2007, 'Bioenergetics in aging: Mitochondrial proton leak in aging rat liver, kidney and heart', Redox Report, vol. 12, no. 1-2, pp. 91-95. https://doi.org/10.1179/135100007X162112
Serviddio G, Bellanti F, Romano AD, Tamborra R, Rollo T, Altomare E et al. Bioenergetics in aging: Mitochondrial proton leak in aging rat liver, kidney and heart. Redox Report. 2007 Feb;12(1-2):91-95. https://doi.org/10.1179/135100007X162112
Serviddio, G. ; Bellanti, F. ; Romano, A. D. ; Tamborra, R. ; Rollo, T. ; Altomare, E. ; Vendemiale, G. / Bioenergetics in aging : Mitochondrial proton leak in aging rat liver, kidney and heart. In: Redox Report. 2007 ; Vol. 12, No. 1-2. pp. 91-95.
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