TY - JOUR
T1 - Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration
AU - Costantini, Marco
AU - Testa, Stefano
AU - Fornetti, Ersilia
AU - Fuoco, Claudia
AU - Sanchez Riera, Carles
AU - Nie, Minghao
AU - Bernardini, Sergio
AU - Rainer, Alberto
AU - Baldi, Jacopo
AU - Zoccali, Carmine
AU - Biagini, Roberto
AU - Castagnoli, Luisa
AU - Vitiello, Libero
AU - Blaauw, Bert
AU - Seliktar, Dror
AU - Święszkowski, Wojciech
AU - Garstecki, Piotr
AU - Takeuchi, Shoji
AU - Cesareni, Gianni
AU - Cannata, Stefano
AU - Gargioli, Cesare
N1 - Funding Information:
We would like to thank Giulio Cossu for critical reading. This study was supported by the National Centre for Research and Development under the frame of BIOMOTION project (PL-TW/VI/3/2019) to W?, the National Science Centre Poland (NCN) within SONATA 14 Project No. 2018/31/D/ST8/03647 to MC, the Italian Ministry of University and Research PRIN Funding Scheme No. 2015FBNB5Y_002 to CG and 201742SBXA_004 to SC, and the Italian Ministry of Defense by the Funding Scheme No. PNRM 2019 for the project RESUMO No. 2018.019 to CG.
Publisher Copyright:
© 2021 The Authors. Published under the terms of the CC BY 4.0 license
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3/5
Y1 - 2021/3/5
N2 - The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture, and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myo-substitutes support the formation of new blood vessels and neuromuscular junctions—pivotal aspects for cell survival and muscle contractile functionalities—together with an advanced muscle mass and force recovery. Altogether, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.
AB - The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture, and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myo-substitutes support the formation of new blood vessels and neuromuscular junctions—pivotal aspects for cell survival and muscle contractile functionalities—together with an advanced muscle mass and force recovery. Altogether, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.
KW - bioprinting
KW - skeletal muscle
KW - stem cells
KW - tissue engineering
KW - VML
UR - http://www.scopus.com/inward/record.url?scp=85101434651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85101434651&partnerID=8YFLogxK
U2 - 10.15252/emmm.202012778
DO - 10.15252/emmm.202012778
M3 - Article
C2 - 33587336
AN - SCOPUS:85101434651
VL - 13
JO - EMBO Molecular Medicine
JF - EMBO Molecular Medicine
SN - 1757-4676
IS - 3
M1 - e12778
ER -